半夏悬浮培养细胞的EMS诱变和耐高温突变体筛选

EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata

目的:确定EMS诱变处理半夏悬浮细胞的最适浓度和处理时间。方法:用0.1%,0.2%,0.4%,0.6%的EMS溶液,处理时间为0.5,1.0,2.0 h,对半夏悬浮细胞系进行诱变处理。结果与结论:结果表明,不同浓度的EMS和不同时间处理半夏悬浮细胞,结果有明显差异,在每个处理时间下,均是随着EMS浓度的增加而悬浮细胞的存活率逐渐下降。EMS诱变半夏悬浮细胞的适宜剂量为0.4%,最佳处理时间为1 h。

Objective： To determine the optimal concentration and processing time of EMS mutation for suspension ceils from Pinellia ternata. Method： Under four EMS concentration gradients （0.1% , 0.2% , 0.4% ,0.6% ） and three processing time gradi- ents （0.5,1.0,2.0 h）, the suspension cells of P. ternata were mutagenized. Result and Conclusion： The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same process- ing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS con- centration for the mutagenesis was 0.4% and the best processing time was 1 hour.