黄连中胆碱的分离及其对小檗碱在HepG2细胞中糖代谢作用的影响

Separation of choline from Coptidis Rhizoma and impact on glucose metabolism of berberine in HepG2 cells

目的:分离黄连亲水性成分,研究其对小檗碱在HepG2细胞中糖代谢作用的影响。方法:黄连亲水性部位高速逆流色谱制备后,硅胶柱色谱法进行分离。MTT法检测HepG2细胞活力,试剂盒检测细胞消耗的葡萄糖。结果:首次从黄连水溶性组分中分离出胆碱。在实验浓度范围内,胆碱能显著增强小檗碱促进细胞吸收葡萄糖的能力,并显著改善小檗碱对细胞生长抑制的影响。与同剂量的小檗碱相比,100 mg.L-1胆碱与38 mg.L-1小檗碱混合后,细胞对葡萄糖的消耗增加34%,存活率上升到75%。结论:胆碱在增强小檗碱促进细胞吸收葡萄糖和减小小檗碱对细胞的毒副作用方面存在显著协同作用。

Objective： To investigate action of hydrophilic constituents from Coptidis Rhizoma on glucose-lowering effect me- tabolism of berberine in HepG2 cells. Method ： Hydrophilic fractions of Coptidis Rhizoma were prepared by high speed counter current chromatography and separated by silica gel column chromatography. MTI＇ assay was used to monitor the proliferation of HepG2 cells, and Kit was used to test the glucose consumption in culture solution. Result： Choline was separated from Coptidis Rhizoma for the first time. Cell assay showed choline can significantly increase the glucose lowering effect of berberine and improve the cytotoxicity of ber- berine within test concentration. Compared with same dose of berberine, berberine 38 mg ·L^-1 in combination with choline 100 mg·L^-1 can make glucose consumption increase by 34% and elevate cell livability up to 75% in HepG2 cells. Conclusion： The results suggest that choline had a synergistic effect on improving glucose absorption of berbcrine and decreasing cytotoxicity of herherinc.