HPLC指纹图谱法分析不同产地藏药甲地然果(花锚)化学成分差异

Difference of chemical constituents contained in Tibetan herb Jiadiranguo from different habitats by HPLC fingerprint

目的:建立藏药椭圆叶花锚药材HPLC指纹图谱,研究椭圆叶花锚成分,比较不同地区椭圆叶花锚异同。方法:采用Welchrom-C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水系统梯度洗脱,检测波长265 nm,流速1.0 mL.min-1,柱温40℃,以指纹图谱软件计算相似度,并进行主成分分析。结果:指认了指纹图谱中12个色谱峰,各批次药材成分种类基本一致,但相似度不高,各批次药材间酮苷与苷元比例差异大,药材成分特征与其产地地理位置间无明显关联。结论:该方法具有简便、专属性强、稳定、可重复的特点,可为椭圆叶花锚药材的鉴别与质量评价提供参考。

Objective ： To establish the HPLC fingerprint for Halenia elliptica herbs, a traditional Tibetan medicine, in order to study constituents contained in H. elliptica from different habitats and compare their differences. Method： HPLC analysis was made on a Welchrom-Cls （4. 6 mm ×250mm,5μm） with water and acetonitrile as mobile phase. The wavelength was detected as 265 nm, the flow rate was 1.0 mL ·min^-1, and the column temperature was 40 ℃. The software for chromatographic fingerprint was applied to an- alyze the similarity. And principal component analysis was conducted. Result： Twelve common chromatographic peaks were identified by fingerprint, showing a low similarity in constituent and variety. The significant difference in the proportion between xanthones and aglycones in each batch of herbs indicated no notable correlation between constituent characteristics and geographic locations of habi- tats. Conclusion： The method is so simple, exclusive, stable and highly repeatable that it can provide reference for identification and quality assessment of H. elliptica herbs.