应用PCR-DNA探针杂交法检测丙型肝炎核糖核酸

PCR-DNA Probe Hybridization Detection of Hepatitis C Virus-RNA

目的探讨PCR-DNA探针杂交法检测丙型肝炎核糖核酸的应用。方法采用PCR-DNA探针杂交法对138例HCVAb阳性者和100例经干扰素治疗的HCVAb阳性者及80例正常者进行对照研究。结果 138例未经治疗的HCVAb阳性者检测一致率为94.2%,100例经干扰素治疗的HCVAb阳性者一致率为95%和80例正常对照测定结果完全一致。结论 PCR-DNA探针杂交法,敏感性高,准确性好,结果易于判定,是较理想的HCV-RNA的检验方法。

Objective To investigate the application of PCR-DNA probe hybridization assay for detecting hepatitis C RNA.Methods 138 cases HCVAb positive and 100 cases treated by interferon HCVAb positive and 80 cases of normal,using the PCR-DNA probe hybridization,were studied as control study.Results The detection of the 138 cases without a ruler consistent rate of 94.2%,100 cases treated with interferon consistent rate of 95%,and 80 cases of normal control results were in agreement.Conclusion PCR-DNA probe hybridization method has high sensitivity,good accuracy.The result is easy to determine.It is an ideal HCV-RNA test methods