摘要
A simple and effective glucose biosensor based on immobilization of glucose oxidase （GOD） in graphene （GR）/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface-controlled process with the large electron transfer rate constant （ks） of 3.42±0.08 s-1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as-prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14mmol·L-1 with a detection limit of 0.03 mmol·L-1. Moreover, it displays a good reproducibility and long-term stability.
A simple and effective glucose biosensor based on immobilization of glucose oxidase （GOD） in graphene （GR）/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface-controlled process with the large electron transfer rate constant （ks） of 3.42±0.08 s-1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as-prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14mmol·L-1 with a detection limit of 0.03 mmol·L-1. Moreover, it displays a good reproducibility and long-term stability.
基金
Acknowledgement This work was supported by the National Natural Science Foundation of China （Nos. 21161003, 20701010）, the Guangxi Natural Science Foundation of China （Nos. 0991093, 2011 GXNSFA018044 and 2010GXNSFF013001） and the S＆T Project of Guangxi Education Department of China （No. 201012MS024）.
