摘要
为了有效利用废弃扇贝壳,提高贝类加工废弃物的附加值,采用双酶法水解猪皮制得胶原多肽,并利用灰化的扇贝壳制备胶原螯合钙。优化的胶原螯合钙制备条件是:pH8,胶原多肽与氯化钙的比为0.3g∶7mL,反应时间50min。经95%乙醇沉淀得到胶原螯合钙产品。用红外光谱法对胶原螯合钙进行表征,结果表明胶原多肽的氨基和羧基都参与了与Ca2+的配位,找到Ca-N伸缩振动峰,说明经酶水解得到的胶原多肽成功螯合了贝壳中的钙离子,使该产品既具有胶原多肽增加骨胶原韧性的作用又具有补钙的特性。
In order to more efficient use of discarded scallop shell and improve the added value of the shellfish processing waste, the collagen polypeptide that maked from pigskin using dual enzyme hydrolysis, was chelated with the calcium of scallop shell. Optimization of the preparation conditions for chelated calcium by collagen was studied. The reaction conditions showed that: pH 8, collagen peptides and calcium chloride ratio of 0.3 g∶7 mL, reaction time 50 min, after 95% ethanol precipitation collagen chelated calcium products. The collagen chelated calcium was characterized by infrared spectrometry, the results show that collagen peptide amino and carboxyl groups are involved in coordination with Ca2+, and found the Ca-N stretching vibration. The result showed that the collagen peptides obtained after enzymatic hydrolysis had successful chelated onto calcium in the shells. So that the product not only has the role of collagen peptides to increase the toughness of bone, but also has the characteristics of calcium.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第5期49-55,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家海洋公益性行业科技专项(No.200805046-4和No.201205031)资助
关键词
扇贝壳
胶原
螯合
钙
红外光谱
表征
scallop shell
collagen
chelated
calcium
Infrared spectrometry
characterization