辛伐他汀对烟雾吸入性损伤大鼠肺的保护作用

Protective effects of simvastatin on smoke inhalation injury in rats lung

目的探讨辛伐他汀对烟雾吸入性损伤大鼠肺的保护作用。方法雄性SD大鼠24只,随机分为正常组、盐水组、辛伐他汀小剂量组、辛伐他汀大剂量组,每组6只。辛伐他汀大剂量组和小剂量组烟雾吸入致伤后,用灌胃器给予辛伐他汀大剂量组50mg/kg辛伐他汀,给予辛伐他汀小剂量组25mg/kg辛伐他汀。盐水组烟雾吸入致伤后灌入等体积的生理盐水,每日一次。正常组不做致伤及灌液处理。四组大鼠分别于48h后取左肺组织行病理切片光镜观察。腹主动脉取血2ml,离心后取血清检测肿瘤坏死因子-α、白细胞介素-6、白细胞介素-10含量,并取右肺组织匀浆取上清液检测髓过氧化物酶、脂质过氧化物酶和超氧化物歧化酶含量。结果光镜下正常组肺泡结构清晰完整,盐水组肺泡腔内可见大量粒细胞聚集、浸润,肺泡间隔增厚,而辛伐他汀大剂量和小剂量组上述病理表现明显减轻。与盐水组相比,辛伐他汀小剂量组肿瘤坏死因子水平降低(P<0.01),脂质过氧化物酶含量降低(P<0.05);辛伐他汀大剂量组肿瘤坏死因子、白细胞介素-6含量降低(P<0.01),白细胞介素-10含量升高(P<0.01),髓过氧化物酶、脂质过氧化物酶水平降低(P<0.05),超氧化物歧化酶水平升高(P<0.05)。结论辛伐他汀可减轻炎症及氧化应激,对烟雾所致吸入性损伤大鼠的肺起保护作用。

Objective To investigate the protective effects of simvastatin on smoke inhalation injury in rats. Methods Twenty-four rats were randomly assigned to four groups ： control group, saline group （ via an orogastric route ） , simvastatin low concentration treated group （25 mg · kg^-1 · d^- 1 , via an orogastrie route） and simvastatin high concentration treated group （50 mg · kg^-1 · d^- 1, via an orogastric route）. Rats were sacrificed at 48 h of the treatments and the trachea and lungs were removed completely. Tissue samples were taken for histopathologic and biochemical analyses. Arterial blood was collected to determine the levels of tumor necrosis factor-or （TNF-a） , interleukin-6 （ IL-6 ） and interleukin-10 （IL-10）. Results Lung malonedialdehyde（MDA） and myelopcroxidase （MPO） levels was significantly decreased （P 〈 0.05）, while superoxide dismutase（SOD） concentration were significantly increased （P 〈0.05 ）in simvastatin high concentration treated group compared with saline group. Also, arterial blood analysis revealed that TNF-α andIL-6 levels were decreased （ P 〈 0. 01 ） , while IL-10 level was significantly increased （ P 〈 0. 01 ）. Conclusions Results of simvastatin administration play a beneficial role in smoke inhalation lung injury to some extent.