摘要
目的:探讨钙超载在急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)发病机制中的作用。方法:采用内毒素(LPS)腹腔注射的方法复制新生大鼠急性肺损伤(ALI)模型,应用荧光测定法检测肺上皮细胞〔Ca2+〕i的水平。结果:ALI组的肺上皮细胞〔Ca2+〕i随着病程的进展呈逐渐升高的趋势,2 h时开始〔Ca2+〕i已显著高于对照组(P<0.05),至8 h时达高峰,之后虽略有下降,但仍明显高于对照组(P<0.05)。结论:肺上皮细胞胞浆内钙超载是导致ALI肺上皮细胞变性坏死的重要机制。
Objective To explore the role of cytosolic calcium overload in pathogenesis of acute lung injury （ALI ） in neonatal rats. Method After endotoxin lipopolysaccharide （LPS） induced acute lung injury （ALl） in neonatal rats, concentrations of calcium in alveolar epithelial cells were determined. Results After treatment with LPS, concentration of calcium in alveolar epithelial ceils increased gradually. The value at 2 hours significantly increased than that of the control group （ P 〈 0. 05 ）. Then it reached peak at 8 hours and de- creased in the following. Conclusion Cytosolie calcium overload maybe the important mechanism for the necrosis of alveolar epithelial cell in acute lung injury.
出处
《吉林医学》
CAS
2012年第16期3365-3366,共2页
Jilin Medical Journal
关键词
急性肺损伤
钙离子
内毒素
新生大鼠
Acute lung injury
Calcium ion
Endotoxin
Neonatal rat
