摘要
[目的]提取和纯化毛蚶(Scapharca subcrenata)中的天然牛磺酸,并对其活性进行研究。[方法]采用薄层色谱法(TLC)对提取的牛磺酸纯度进行分析,并对其结构用红外光谱(IR)和核磁共振波谱(NMR)进行鉴定,然后对其细胞毒活性进行检测。[结果]毛蚶富含牛磺酸,对人白血病细胞HL-60、肝癌细胞SMMC-7721、肺癌细胞A-549、乳腺癌细胞MCF-7和结肠癌细胞SW480没有明显的细胞毒活性。[结论]该研究为天然牛磺酸的开发和利用提供了依据。
[ Objective 3 The aim was to isolate and purify taurine in Scapharca subcrenata, and its activity was studied. [ Method ] The purity of isolated taurine was analyzed by TLC, and its structure was determined by IR and NMR, and then its cytotoxicity in vitro was detected. [ Result] The results showed that the yield of taurine in S. subcrenata was high, and there were no significant cytotoxic activities against human leukemia cell line HL-60, hepatocarcinoma cancer cell line SMMC-7721, lung cancer cell A-549, human breast cancer cell line MCF-7 and colorectal cancer cell line SW480. [ Conclusion ] The study provides a basis for development and utilization of nature taurine.
出处
《安徽农业科学》
CAS
2012年第17期9288-9289,共2页
Journal of Anhui Agricultural Sciences
基金
广西科学研究与技术开发计划项目(桂科攻11107011-8)
关键词
毛蚶
牛磺酸
提取
细胞毒性
Scapharca subcrenata
Taurine
Isolation
Cytotoxicity
