细枝木麻黄小枝多酚提取液的抗氧化活性研究

Antioxidative Effects of Polyphenol Extracts from Casuarina cunninghamiana Miq. Branchlet

[目的]研究细枝木麻黄小枝多酚提取液的抗氧化活性。[方法]以细枝木麻黄小枝为原料,以乙醇为提取溶剂,采用超声波辅助法提取细枝木麻黄多酚,并研究细枝木麻黄小枝多酚提取液的体外抗氧化作用及其对玉米油的抗氧化性能的影响。[结果]体外抗氧化试验结果表明,细枝木麻黄小枝多酚浓度为0.90 mg/ml时,对DPPH.的清除率为95.4%,半清除率(IC50)为68 mg/L;浓度为1.20mg/ml时,对.OH的清除率达99.0%,IC50为74 mg/L;浓度为1.20 mg/ml时,对O2-.的清除率达98.9%,IC50为240 mg/L。对玉米油脂抗氧化性影响的试验结果表明,贮藏10 d,空白对照过氧化值(POV)82.78 mmol/kg;添加0.016%细枝木麻黄多酚时,POV值为41.73mmol/kg。[结论]细枝木麻黄多酚体外可清除DPPH.、.OH和O2-.,具有较强的综合抗氧化性能。

[ Objective ] Study the antioxidative effects of polyphenol extracts from Casuarina cunninghamia＇na Mig. Branchlet. [ Method ] The poly- phenol was extracted from Casuarina cunninghamiana Miq. Branchlet ,using ethanol as extractant and employing ultrasonic treatment. In the mean time,the effects of polyphenol extracts were investigated both antioxidation in vitro and the peroxide value （POV） of maize oil. [ Result] The scavenging activity of polyphenol extracts was 95.4% for DPPH · ,the scavenging concentration of polyphenol extracts was 68 mg/L for 50% DP- PH ·,when the concentration of polyphenol extracts was 0.90 mg/ml. The scavenging activity of polyphenol extracts was 99.0% for · OH, the scavenging concentration was 74 mg/L for 50% ~·OH,when the concentration was 1.20 mg/ml. The scavenging activity was 98.9% for superox- ide anion radicals （O^2-· ） ,the scavenging concentration was 240 mg/ml for 50% O^2- · ,when the concentration was 1.20 mg/ml. The experi- mental results of the effects on the POV in maize oil showed that the POV was 82.78 mmol/kg when maize oil was layed in for 10 days without the polyphenol ,while the POV was 41.73 mmol/kg with 0. 016% polyphenol. [ Conclusion] Polyphenol extracts from Casuarina cunninghamiana Mig. Branchlet could scavenge DPPH ·,· OH and O^2- ·,which had integrated antioxygenic property.