花脸香蘑酯酶同工酶分析

Study on Esterase Isozyme of Lepista sordida

[目的]对花脸香蘑酯酶同工酶进行分析,以期为花脸香蘑酶谱分析奠定基础。[方法]采用聚丙酰胺凝胶电泳方法,对7种不同提取液提取的酯酶进行分析,探讨不同提取液对酯酶同工酶提取效果的影响,找出最优提取液,并分析不同培养方法和组织对同工酶的影响。[结果]7种不同提取液提取的酯酶酶带保持一致,但提取效果各异,用pH 8.0、0.1 mol/L Tris-HCl缓冲液提取时效果较好。液体菌丝体、试管种、驯化花脸香蘑菌盖酶带均有5条;野生花脸香蘑菌盖和菌柄只有3条酯酶同工酶谱带;野生花脸香蘑菌盖与驯化花脸香蘑菌盖的酯酶谱带条数不同。因此在选用酯酶同工酶作为遗传指标,用作花脸香蘑液体菌种鉴定时,必须注意保持培养基、培养条件以及取材部位的一致性。[结论]该研究结果为花脸香蘑酶谱分析奠定了基础。

[ Objective] To analyze the estcrase isozyme of Lepista sordida, so as to lay foundation for its zymogram analysis. [ Method ] The esterase isozyme was extracted by polyacrylamide gel electrophoresis in seven different kinds of buffer, the extraction results were compared to screen oat the best buffer, and the effects of different culture methods and tissues on esterase isozyme were studied. [ Result ] Although the bands of esterase isozyme were the same in seven kinds of buffer, their extraction results were different. The extraction of esterase isozyme was the best under 8.0 pH and 0.1 mol/L Tris-HCL. The number of bands was five in liquid mycelia, test tube mycelia, pileus of domesticated Lepista sordida, but only three in pileus and stipe of wild Lepista sordida, so the medium, culture conditions and sample positions should be the same to identify liquid fungus with esterase isozyme. [ Conclusion] The study results lay foundation for the zymogram analysis of Lepista sordida.