摘要
[目的]研究药用植物灵菊七(Gynura medica)的细胞染色体特点,并对其染色体核型进行分析。[方法]以灵菊七幼苗茎尖为材料,从上午6:30～10:30,每隔20 min取材一次,采用常规制片技术制片,显微镜观察中期分裂相细胞,对染色体完全分散开的细胞进行染色体计数及核型分析。[结果]在染色体分散良好的102个中期分裂相细胞中,有96.08%含20条染色体,3.92%含40条染色体;同时结果表明上午7:50～9:30取材较佳。[结论]G.medica为二倍体植物,体细胞含有10对染色体,核型公式为2n=2x=20 m,染色体核型属于1A型。该研究结果为国内首次报道。
[ Objective ] To study the chromosomal characteristics and karyotypes of G. medica. [ Method ] Stem tip of G. medica seedlings were collected every 20 min from 6：30 to 10：30 am, and all materials were treated and squashed for investigating chromosomal number and karyotypes analysis. [ Result] In total 102 stem tip cells in metaphase, 96.08% of cells are with 20 chromosomes, and 3.92% of cells are with 40 chromosomes. The optimal time for sampling was from 7：50 to 9：30 am. [ Conclusion] G. medica is the diploid plant with 20 chromo- somes. Karyotype analysis gets formula 2n = 2x = 20 m, and all 10 pairs of chromosomes belong to type 1A. The article reported the chromo- somes characteristics of G. medica for the first time in China.
出处
《安徽农业科学》
CAS
2012年第18期9644-9645,9650,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省中医药科技计划项目(2011ZB132)
浙江省大学生科技创新计划项目(ZJRCKJCX201007)
关键词
灵菊七
染色体
核型
Gynura medica
Chromosome
Karyotype
