杜仲叶提取物对力竭运动及恢复小鼠肝脏和骨骼肌的保护作用

Protective Effects of Extracts from Eucommia ulmiodes Olirer Leaves on Liver and Quadriceps Femoris Tissue of Mice at Exhaustive Exercise and Recovery

[目的]研究杜仲叶提取物对力竭运动及恢复小鼠肝脏、股四头肌中SOD、POD、MDA和血清中AST、ALT、AKP活性的影响。[方法]取72只ICR小鼠,随机分为安静对照组A、安静杜仲叶提取物组B、力竭运动对照组C、力竭运动杜仲叶提取物组D、力竭运动恢复对照组E和力竭运动恢复杜仲叶提取物组F,每组12只,各给药组小鼠每日按1.5 g/kg剂量以杜仲叶提取物灌胃,各对照组小鼠以蒸馏水灌胃,连续30 d后,将运动组小鼠置于水中游泳至力竭,记录力竭运动时间;C组和D组小鼠运动后即刻摘眼球采血,并取肝脏和股四头肌备用。A组和B组不作运动处理,直接摘眼球采血,并取肝脏、股四头肌备用。E组和F组小鼠恢复24 h后摘眼球采血,并取肝脏、股四头肌备用;测定小鼠肝脏、股四头肌MDA含量、SOD和POD活性及血清中AST、ALT和AKP活性。[结果]杜仲叶提取物可以提高力竭运动状态下小鼠肝脏和股四头肌中SOD和POD活性,减少MDA生成,并可明显降低血液中AST、ALT和AKP的活性。[结论]杜仲叶提取物对力竭运动小鼠的肝脏和骨骼肌有保护作用,并具有促进机体恢复的作用。

[ Objective ] To study the effects of extracts from Eucommia ulmiodes Olirer leaves （EEUOL） on the levels of SOD ,. POD and MDA in liver and quadriceps femoris tissue and the levels of AST, ALT and AKP in serum of mice at exhaustive exercise and recovery. [ Method] A total of 72 ICR mice were randomly divided into 6 groups of 12 each ： quiet control group （ A）, quiet EEUOL group （ B）, exhaustive exer- cise control group （C）, exhaustive exercise EEUOL group （D）, exhaustive exercise recovery control group （E） and exhaustive exercise re- covery EEUOL group （F）. Mice in drug groups were given EEUOL at the dosage of 1.5 g/kg per day for successive 30 days by intragastric administration, while those in control groups were given distilled water. The exhaustive exercise time of mice in exercise groups was recorded when they swam in the water until exhaustion. After exercise, the blood was immediately sampled from eyeball of mice in groups C and D, the liver and quadriceps femoris tissue were taken from their bodies for later use. Because of mice in groups A and B were not given exercise treat- ment, the blood was directly sampled from eyeball, and the liver and quadriceps femoris tissue were taken from their bodies. As to groups E and F, the blood was sampled from eyeball after 24 h recovery, and the liver and quadriceps femoris tissue were taken from their bodies. Spec- trophotography was used to measure the contents of MDA, the activity of SOD and POD in liver and quadriceps femoris tissue, and the activity of AST, ALT and AKP in serum of mice at exhaustive exercise and recovery. [ Result] EEUOL could increase the activities of SOD and POD in liver and quadriceps femoris tissue of mice at exhaustion and reduce the levels of MDA in liver and quadriceps femoris tissue of mice at ex- haustive exercise and recovery. EEUOL could also reduce the activities of AST, ALT and AKP in serum mice at exhaustive exercise and recov- ery. [ Conclusion] Extracts from Eucommia ulmiodes Olirer leaves have a protective effect on liver and quadriceps femoris tissue of mice at exhaustive exercise, and have an accelerated effect on mice recovery.