酶联免疫法检测猪肝中β-兴奋剂残留的不确定度分析

Uncertainty Analysis of β-agonists in Pork Liver by ELISA

[目的]分析酶联免疫法检测猪肝中β-兴奋剂的不确定度,探讨各因素对检测结果的影响。[方法]参考JJF1059-1999《测量不确定度评定与表示》和CNAS-GL06《化学分析中不确定度的评估指南》规定的基本方法和程序,分析了影响测量不确定度的来源,并对各不确定度分量进行了评估。[结果]当猪肝中β-兴奋剂含量为187.64 ng/kg时,其扩展不确定度为6.68 ng/kg(k=2)。[结论]影响检测结果不确定度的主要因素为回收率和标准曲线拟合,在实际测量过程中严格控制试验条件可提高酶联免疫检测的准确性和可靠性。

[ Objective ] A method for uncertainty analysis of β-agonists in pork liver by ELISA was established to investigate the effects of each factor on the results. [ Method] According to the basic method and procedure for uncertainty assessment given in JJF1059-1999 Evaluation and Expression of Uncertainty in Measurement and CNAS-GL06 Guidance on Evaluating the Uncertainty in Chemical Analysis, the sources of uncer- tainty for determination were analyzed and each component of uncertainty was evaluated. [ Result] The content ofβ-agonists in pork liver was 187.64 ng/kg and the expanded uncertainty was 6.68 ng,/kg（k =2）. [ Conclusion] The major affecting factors of determination results were coefficient of recovery and standard curve fitting. The experimental condition of practical measurement should he controlled strictly to improve the accuracy and reliability of ELISA determination.