ILK反义寡核苷酸抗人上皮性卵巢癌移植瘤的体内实验研究

An in vivo study on the effect of ILK antisense oligonucleotide on anti-xenograft of human epithelial ovarian cancer

目的通过整合素连接激酶(integrin linked kinase,ILK)反义寡核苷酸(antisense oligonucleotide,ASODN)转染,来探讨ILK-ASODN对人上皮性卵巢癌裸鼠皮下移植瘤形成的抑制作用,及对已形成的移植瘤的治疗作用。方法研究分为两部分:第一部分将转染了ILK-ASODN的实验组和未转染ILK-ASODN的对照组的HO-8910细胞分别接种于裸鼠皮下,观察裸鼠体重变化、肿瘤出现的时间、肿瘤体积及重量的变化,并计算抑瘤率;第二部分将HO-8910细胞直接接种于裸鼠皮下,建立人卵巢癌裸鼠模型,并将裸鼠随机分为对照组和治疗组,每次腹腔注射治疗后称量裸鼠体重、肿瘤体积及重量,并计算抑瘤率。结果第一部分研究的实验组裸鼠肿瘤出现时间明显晚于对照组、肿瘤体积及重量增长速度显著减缓,与对照组比较差异在统计学上均具有极显著意义(P<0.01);第二部分研究的治疗组裸鼠肿瘤体积和重量增长速度受到明显抑制,与对照组比较差异在统计学上均具有极显著意义(P<0.01)。结论 ILK-ASODN对裸鼠皮下移植瘤的形成具有明显抑制作用;并能明显抑制已成瘤裸鼠体内的肿瘤生长,表明对人卵巢癌具有一定的治疗作用。

Objective To explore the inhibiting of transfection of integrin - linked kinase antisense oligonucleotides （ ILK - ASODN） on the development of subcutaneous xenograft of human epithelial ovarian cancer in nude mice, and therapeutic effect on treatment of the developed subcutaneous xenograft. Methods This study included two parts. The first part was subcutaneous inoculation of human ovarian cancer cell lines （ HO - 8910） with transfection of ILK - ASODN to the nude mice in the experimen- tal group, and subcutaneous inoculation of HO - 8910 without transfection of ILK - ASODN to the nude mice in the control group to observe the changes of weight of nude mice, time for tumor development, changes of tumor volume and weight, and calculated the inhibitory rates. The second part was subcutaneous inoculation of the HO -8910 ceils in nude mice to establish the model of human ovarian cancer in nude mice. Then the mice were randomly divided into treatment group and control group. After each in- traperitoneal injection of the treatment, the weights of the mice, the weight and volume of the tumors in nude mice were recorded, and the rates of tumor inhibition were calculated. Results In comparison with the control group, tumors of the nude mice in the exper/mental group of the first part of the study developed statistica/ly significant later （ P 〈0.01） and grew statistically signifi- cant slower in volume （ P 〈0.01） and in weight （ P 〈0.01）. In comparison with the control group, tumors of the nude mice in the treatment group of the second part of the study grew in volume and weight much slower, showing statistically significant （ P 〈 0.01）. Conclusion Our study showed ILK - ASODN significantly inhibited the development, and the growth of the developed xenografts of human ovarian cancer in nude mice, indicating a preventive and therapeutic effect.