定向结构组织工程软骨支架的构建

Fabrication of Oriented-structure Scaffold for Cartilage Tissue Engineering

目的基于仿生学原理,构建类似软骨真实结构的定向结构组织工程软骨支架,以解决目前组织工程软骨修复过程中存在的支架亲水性相容性不够的缺陷以及新生软骨生理功能和机械性能不能长期维持等问题。方法选择软骨基质及壳聚糖共混溶液基于定向结晶及热致相分离工艺构建定向结构支架,研究支架孔径及力学性能生物相容性、细胞生长情况等。结果材料配比、模具底端温度等是影响着定向结构支架的孔径及力学性能的主要因素,定向结构支架轴向力学性能优于垂直轴向,细胞大量贴附支架表面,并沿着定向孔隙生长。结论采用上述方法构建的支架孔径可控,轴向抗压性能好,生物相容性,亲水性好,并有利于软骨细胞沿支架定向孔隙排布。

Objective To fabricate an oriented-structure scaffold similar to real cartilage structure according to bionics principles, so as to solve the following two main problems in cartilage repair with tissue engineering methods： （i） insufficient hydrophilicity and biocompatibility （ii）the physiological function, mechanical ca- pacity of tissue engineering cartilage can＇ t sustain over longer period of time. Methods Cartilage extracellular matrix and ehitosan were used to fabricate oriented-structure scaffold based on crystallographic orientation and thermally induced phase separation. Then the pores structure and mechanical performance, basic biological properties of scaffolds were studied. Results Parameters of material proportion and mould bottom temperature could affect the scaffolds pores structure and mechanical performance of oriented-structure scaffold. The me- chanical performance of the oriented-structure along the axis was better than that along the vertical direction. Cells could congregate and proliferate along oriented pores. Conclusion Pore size and mechanical property of cartilage scaffold fabricated by the above method is controllable. The scaffold has good pressure resistance along axis, good biocompatibility and hydrophilicity. It is suitable for cells to congregate and proliferate along oriented pores.