非侵入性聚焦超声融脂量效动物实验的组织学观察

Histological observation on doses-effects relationship of non-invasive focused ultrasound irradiation on fat in pigs

目的:观察猪皮下脂肪经不同剂量非侵入性聚焦超声照射后的病理学变化。方法:采用由功率0 W、450 W、550W、650W;治疗次数1、2、3组成的9个实验组和1个零剂量对照组,频率1.9MHz的聚焦超声,非侵入照射猪背部皮肤,即刻切取包括皮肤全层并深达肌肉浅层的标本块制作病理切片,HE染色,组织学观察。结果:各实验组脂肪层内,显微镜下可见大量点状脂肪细胞损伤灶。在低、中、高输出功率组,灶内细胞损伤数分别为1～5个、2～10个和15～40个。损伤灶内中央的脂肪细胞消失,边缘的脂肪细胞部分破裂,小血管保存完好,无游离红细胞。未见炭化、组织凝固现象,未见皮肤、肌膜与骨骼肌细胞损伤。结论:1.9MHz非侵入聚焦超声能选择性破坏脂肪细胞,脂肪细胞损伤程度与设备输出功率和照射次数有正相关,具有皮肤、小血管、筋膜等组织的安全性。

Objective To observe the pathological changes of fat in pigs irradiated by non-invasive focused ultrasound in different doses. Methods A non-invasive focused ultrasound with a frequency of 1.9MHz was used in this experiment to six pigs.The back skin in each pig was irradiated with different power at 0W,450W,550W,660W and 1,2,3times,which produced 9 different lesioning data groups and 1 normal control group. After radiations the full- thickness skin and muscle specimens were immediately cut off and the pathological sections were made. Results A large number of damage foci of fat cells can be seen in all experimental groups under the microscope. The stove cell were in the number of 1-5 in low output power groups, in the number of 2~10 in medium and in the number of 15~40 in high. The damaged fat cells in the center of foci were disappeared,and the edge were broken. The small blood vessel were intact, and there were no red blood cells seen in the foci. There were no the carbonized,coagulated tissues in the experimental areas. Conclusion Non-intrusive focused ultrasound with a frequency of 1.9MHz can be selected to destroy fat cells.There were positive relationship between the degrees of damage with output power and treatmental times.It is security with the skin, small blood vesse and fascia.