S100A2在围着床期小鼠子宫内膜表达的动态观察

Spatiotemporal expression of S100A2 in peri-implantation mouse endometrium

目的:了解S100A2 mRNA及蛋白在围着床期小鼠子宫的表达情况,为阐明S100A2在胚胎着床过程中的作用积累实验依据。方法:应用荧光定量PCR(FQ-PCR)和免疫组化技术分别检测S100A2 mRNA和蛋白的表达情况。结果:①S100A2mRNA在妊娠第1～6天子宫中均有表达,且表达量逐渐降低;同时,在第5天,着床点的含量明显低于非着床点。②S100A2蛋白主要表达于小鼠子宫内膜基质细胞的胞浆中,其表达量在第1、2天很丰富,第3、4、5天很低。③子宫内膜的腔上皮和腺上皮细胞未见S100A2表达。结论:在胚胎着床过程中,S100A2在小鼠子宫中的表达呈动态变化,提示可能参与了子宫内膜对滋养层细胞侵袭的调控。

Objective：To identify the expression pattern of S100A2 mRNA and protein in peri-implantation endometrium in mice,and to accumulate experimental proof which S100A2 might play roles in embryo implantation.Methods：mRNA and protein expression of S100A2 were detected by fluorescence quantitative polymerase chain reaction（FQ-PCR） and immunohistochemistry.Results：① S100A2 mRNA was steadily expressed in the mouse uterus during d1-d6 of pregnancy,and its quantity gradually decreased day by day.On d5 of pregnancy,its expression level was lower in the implantation sites than that in inter-implantation sites.②S100A2 protein was localized in cytoplasm of mouse endometrial stromal cells,while its level dynamically changed：strongly positive on d1-d2,weakly positive on d3-d5.③No expression was detected in endometrial luminal epithelial cells and gland cells.Conclusion：Dynamic expression of S100A2 in mouse uterus during embryo implantation shows that it might play roles in regulation of embryo invasion into the uterine endometrium.