摘要
为了解虎红平板凝集试验(RBPT)、试管凝集试验(SAT)和酶联免疫吸附试验(ELISA)三种血清学方法在奶牛布氏杆菌病诊断方面的实际应用情况,本试验采集了10份疑似奶牛布氏杆菌病的血清样本,同时采用上述三种血清学诊断方法进行了对比研究。结果显示,RBPT、SAT和ELISA的阳性检出率分别为70%、80%和90%,以ELISA的阳性检出率为最高。因此,具备特异、灵敏、快速等优点的ELISA血清学诊断方法更适合奶牛布氏杆菌病的抗体检测及其研究。
In order to know the application of RBPT, SAT and ELISA in serodiagnosis of cows brinell bacillus disease, the 10 serum samples collected from suspected cases were detected and analysed by the test methods above. The results showed that the positive rate of RBPT, SAT and ELISA were 70%, 80% and 90%, respectively. So, ELISA which has high specificity, sensitivity and speed is more suitable for antibody detection and research of brucellosis in dairy cattle.
出处
《中国奶牛》
2012年第11期29-31,共3页
China Dairy Cattle
