Detection of ＂ Candidatus Phytoplasma asteris＂ in Brussels Sprout and Its Possible Association with Flower Bud Failure in Poland
Detection of ＂ Candidatus Phytoplasma asteris＂ in Brussels Sprout and Its Possible Association with Flower Bud Failure in Poland
摘要
Severe growth abnormalities including shoot stunting, leaf blade reduction and flower bud failure of Brussels sprout were observed in Poland. The presence of phytoplasma in diseased as well as in healthy looking plants, was demonstrated by nested polymerase chain reaction assay employing phytoplasma universal rRNA primer pairs-P1/P7 followed by R16F2n/R16R2. Products of PCR primed by R 16F2n/R 16R2 primer pair from naturally infected Brussels sprouts were sequenced. Comparison of the obtained 16S rDNAs revealed high nucleotide sequence identity between analyzed phytoplasma isolates （99.8%-100%）. They were also nearly identical with the sequences of other phytoplasmas isolates from sub-group 16SrI-B, and they were classified as members of ＂Candidatus Phytoplasma asteris＂.
参考文献32
-
1E. Seem011er, C. Marcone, U. Lauer, A. Ragozzino, M. G~schl, Current status of molecular classification of the phytoplasmas, Journal of Plant Pathology 80 (1998) 3-26.
-
2I.M. Lee, R.E. Davis, D.E. Gundersen-Rindal, Phytoplasmas: Phytopathogenic mollicutes, Annual Review of Microbiology 54 (2000) 221-255.
-
3A. Bertaccini, R.E. Davis, I.M. Lee, Distinction among mycoplasmalike organisms (MLOs) in Gladiolus, Ranunculus, Brassiea and Hydrangea through detection with nonradioactive cloned DNA probes, Phytopathologia Mediterranea 29 (1990) 107-113.
-
4C. Marcone, A. Ragozzino, E. Seemtiller, Detection and identification of phytoplasmas infecting vegetable, ornamental, and forage crops in southern Italy, Journal of Plant Pathology 79 (1997) 211-217.
-
5C. Marcone, A. Ragozzino, Detection of phytoplasmas in Brassica spp. in southern Italy and their characterization by RFLP analysis, Zeitschrift ft~r Pflanzenkrankheiten und Pflanzenschutz 102 (1995) 449-460.
-
6C. Marzachi, A. Alma, M. d'Aquilio, G. Minuto, G. Boccardo, Detection and identification of phytoplasmas infecting cultivated and wild plants in Liguria (Italian Riviera), Journal of Plant Pathology 81 (1999) 127-136.
-
7M. Vibio, A. Bertaccini, I.M. Lee, R.E. Davis, M.F. Clark, Differentiation and classification of aster yellows and related European phytoplasmas, Phytopathologia Mediterranea 35 (1996) 33-42.
-
8A. Bertaccini, Z. Vorackova. M. Vibio, J. Franova, M. Navratil, J. Spak, J. Nebesarova, Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group, Plant Pathology 47 (1998) 317-324.
-
9M. Starzycki, E. Starzycka, Parents and progeny plant deformation of Brassica napus L. infected by Phytoplasma sp., Rogliny Oleiste-Oilseed Crops 21 (2000) 399-408.
-
10V.I. Maliogka, J.T. Tsialtas, A. Papantoniou, K. Eflhimiou, N.1. Katis, First report of a phytoplasma associated with an oilseed rape disease in Greece, Plant Pathology 58 (2009) 792.
-
1Zhao Hanqing,Li Shaoli,Cao Ling,Yuan Yi,Xue Guanhua,Feng Yanling,Yan Chao,Wang Liqiong,Fan Zhaoyang,Sun Hongmei.Surveillance of Mycoplasma pneumoniae infection among children in Beijing from 2007 to 2012[J].Chinese Medical Journal,2014(7):1244-1248. 被引量：65
-
2冯华,张改平,郭军庆,王爱萍,赵丽娜,杜晓明,王林林.对虾白斑综合征病毒LAMP检测方法的建立[J].中国兽医科学,2011,41(7):707-711. 被引量：6
-
3查红波,王双山,徐镔蕊,欧阳素贞,靳玉芬,刘尚高.我国猪场常见免疫失败的影响因素分析[J].畜牧兽医科技信息,2003,19(5):20-23. 被引量：3
-
4Min Zhao,Wen-Jing Zhu,Yuan Qian,Yu Sun,Ru-Nan Zhu,Jie Deng,Fang Wang,Ya-Xin Ding,Run Tian,Chuan-He Liu,Ling-Hui Meng,Lin-Qing Zhao.Association of Different Human Rhinovirus Species with Asthma in Children： A Preliminary Study[J].Chinese Medical Journal,2016(13):1513-1518. 被引量：4
-
5柴下,陆宏达,刘俊杰,岳蒙蒙,卢德刚.武汉单极虫巢式聚合酶链反应检测方法的建立与优化[J].上海海洋大学学报,2014,23(4):556-563. 被引量：3
-
6杨俊秀,张刚龙,樊军锋,周永学,王培新,李有忠,李文爱.泡桐属不同种和种源对丛枝病抗性调查[J].西北林学院学报,2005,20(4):115-116. 被引量：5
-
7病原体改变植物气味吸引昆虫——新发现有助于研制出新的植物抗虫方法[J].昆虫知识,2009(1):2-2.
-
8Jankowski Kazimierz Kolczarek Roman Jankowska Jolanta Sosnowski Jacek.The Strategies of Grassland Management in Farms of Northeastern Part of Poland[J].Journal of Life Sciences,2013,7(7):727-731.
-
9R.PASTORELLI,R.PICCOLO,S.SIMONCINI,S.LANDI.New Primers for Denaturing Gradient Gel Electrophoresis Analysis of Nitrate-Reducing Bacterial Community in Soil[J].Pedosphere,2013,23(3):340-349. 被引量：2
-
10李巍,张西臣,李建华,李淑红,宫鹏涛,杨举.应用巢式PCR-RFLP方法鉴别我国常见的几种隐孢子虫[J].吉林农业大学学报,2011,33(1):69-73. 被引量：5
