西藏不同区域砂生槐在不同采收时间的花、茎、叶及种子中苦参碱、氧化苦参碱含量比较

Comparing Sophocarpidine and Ammothamnine contents in flower,stem,leaf and seed of Sophora at different harvesting time and different region in Tibet

目的:测定西藏不同区域产砂生槐在不同采收时间花、茎、叶及种子中的苦参碱与氧化苦参碱的含量,为其合理采收利用提供科学依据。方法:使用高效液相色谱(HPLC):SHIMADZU C18色谱柱(150×4.6);流动相:甲醇(0.05mol/L)-磷酸二氢钠溶液(含磷酸2ml/L)-高氯酸钠(15ml:85ml:0.5g),检测波长为215nm,流速为0.7ml/min,柱温为室温。结果:种子中氧化苦参碱、苦参碱含量较高,最高的为日喀则七月份采种子,苦参碱含量(24.48mg/g),氧化苦参碱含量(51.125mg/g)。结论:砂生槐花、茎、叶及种子中均含有苦参碱和氧化苦参碱,不同区域、不同采收时间其含量有差异,种子采收7月份最佳,利用叶子应在6-7月。

In the present paper, the content of sophocarpidine and ammothamnine in flowers, stems, leaf and seeds of Sophora collected at different time in different regions in Tibet were measured to provide scientific basis for reasonable harvesting and use of recourse by using High Performance Liquid Chromatogram （（HLPC） SHIMADZUC18 chromatic column 150×4.6, mobile phase including colonial spirit （0.05mol/L）, sodium dehydrogen phosphate solution （phosphoric acid 2ml/L）, Sodium perchlorate （15ml： 85ml：0.5g）, and 215nm of detecting wavelength, 0.Tml/min of velocity rate at room temperature. The results showed the highest content of ammothamnine and sophocarpidine are in seed collected in July from Shigaze and the content of sophocarpidine and ammothamnine are difference between regions and at different collecting times.