摘要
目的:建立高效液相色谱法测定二十五味珊瑚丸中西红花苷含量的方法。方法:采用HPLC法,色谱柱:Dionex C18柱(250mm×4.60mm,5um);流动相为甲醇-水(52:48),流速为0.8ml/min,检测波长为440nm,柱温为25℃;进样量10μl;用标准曲线法定量,测定二十五味珊瑚丸中西红花苷含量。结果:西红花苷Ⅰ在33μg～330μg范围内与峰面积呈良好的线性关系(r=0.9999),西红花苷Ⅱ在62μg～310μg范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率为103.73%,RSD为1.68%(n=6)。结论:本方法操作简单、重现性好,可用于测定二十五味珊瑚丸中西红花苷Ⅰ&Ⅱ含量。
The content of crocin in the 25 odors Coral pill were quantitatively measured with standard curve by using HPLC （chromatographic column： Dionex C18 （250mm×4.60mm, 5urn）; mobile phrase consisted of MeOH-water （52：48）;flow rate： 0.8ml/min; detection wavelength： 440nm; column temperature： 25%; sample load： 10μl）to establish a determining method of the crocin [ ＆ Ⅱ in the 25 odors Coral pill. The results showed that there is a good linear relationship between peak area and in the range of 33-330μg （r=0.9999） of crocin and between peak area in the range of 62-310μg （r=0.9998） of crocin at a mean recovery rate of 103.73% and 1.68% of RSD （n=6）. In conclusion, this method could be used to determine the crocin in the 25 odors Coral pill with simple operation and good repeatability,
