不同发酵条件下Tween80对乳酸链球菌SM526产Nisin的影响

Effect of Tween8O on the Growth of Lactococcus lactis SM526 and Nisin Activity

以ＳＹＳ３为发酵培养基，研究了在不同发酵条件下Ｔｗｅｅｎ８０对乳酸链球菌ＳＭ５２６ 产Ｎｉｓｉｎ的影响．在自然ｐＨ、兼性厌氧发酵条件下，在体积浓度０～１％的范围内，乳链菌 肽的活性随Ｔｗｅｅｎ８０浓度的提高而提高，最适浓度（１％）时，提高幅度为３６％；浓度超过 １％后， Ｎｉｓｉｎ的活性有所降低．在表性厌氧条件下通过流加 ＮａＯＨ溶液保持恒定 ｐＨ为 ６．５并添加 １％的 Ｔｗｅｅｎ８０进行发酵，同不添加 Ｔｗｅｅｎ８０相比，发酵单位提高了 ３０％．在 厌氧条件下恒定ｐＨ为 ６．５并添加１％的 Ｔｗｅｅｎ８０进行发酵，同不添加 Ｔｗｅｅｎ８０相比，发 酵单位提高了１７％

Using SYS3 as fermentation medium, the influence of Tween80 concentration on nisin activity during fermentation by Lactococcus lactis SM526 was studied. The results showed that nisin activity was enhanced with the Tween80 concentration increased from 0 to 1% ( ), but the activity was reduced when the concentration was over 1%. Under the natural pH and facultative anaerobic conditions, the optimum concentration of Tween80 was 1%, at which nisin activity increased by 36% above that without Tween80 addition. The nisin activity increased by 30% (in facultative anaerobic media) and 17% (in anaerobic media ) at constant pH 6.5 with Tween80 concentration of 1% as compared to those without Tween80 in the medium.