家兔脱髓鞘动物模型的建立

Establishment of Rabbit Model of Demyelination

目的探讨建立新西兰大白兔脱髓鞘的动物模型。方法取新西兰大白兔10只制备脊髓匀浆。将20只新西兰大白兔按随机数字表法分为2组。正常对照组(n=10)用PBS代替免疫原做成匀浆进行注射,实验组(n=10)后足垫一次性皮下注射混匀的匀浆1.5mL。观察2组新西兰大白兔的体质量及下肢痛阈、坐骨神经髓鞘的组织病理的改变等情况。结果实验组体质量从致敏后至恢复期明显减轻。实验组发病高峰期(28d)、恢复期(40d)体质量均较正常对照组明显减轻(均P<0.01)。实验组发病初期(21d)、发病高峰期(28d)下肢痛阈值均较正常对照组明显下降(P<0.05或P<0.01),实验组恢复期(40d)下肢痛阈值与正常对照组比较差异无统计学意义(P>0.05)。结论运用接种同种异体脊髓匀浆加弗氏佐剂可成功制备家兔外周神经脱髓鞘动物模型。

Objective To establish a New Zealand white rabbit model of demyelination.Methods Spinal cord homogenate was obtained from 10 New Zealand white rabbits.In addition,20 New Zealand white rabbits were randomly divided into two groups,with 10 rabbits in each group.The normal control group（n=10） was injected with PBS homogenate instead of immunogen.The experiment group was subcutaneouly injected with spinal cord homogenate（1.5 mL） in each hind foot pad at one time.Body mass,lower limb pain threshold and histopathological changes in sciatic nerve myelin were observed in both groups.Results In experiment group,body weight was significantly reduced from sensibilization to convalescence.Compared with normal control group,spinal cord homogenate significantly mitigate body mass in the early stages of the onset（21 days）,during peak incidence period（28 days） and during recovery period（40 days）（all P0.01）,but obviously decreased lower limb pain threshold in the early stages of the onset and during peak incidence period（P0.05 or P0.01）.There recovery period（40 days） was no significant difference in pain threshold between the two groups（P0.05）.Conclusion The inoculation with allogenic spinal cord homogenate and Freund＇s adjuvant can be used to establish rabbit model of demyelination.