醇提蛋黄油治疗烫伤的药效学探讨

Pharmacodynamic study on scald wound of egg yolk oil by ethanol extract

目的观察醇提蛋黄油促进烫伤创面愈合的作用及其抗炎、抑菌效果。方法选用健康昆明种小鼠随机分为烫伤对照组、湿润烧伤膏对照组、干馏蛋黄油治疗组和醇提蛋黄油治疗组,建立小鼠背部Ⅱ度烫伤模型,观察药物对烫伤的愈合时间,考察药物的治疗效果;通过对二甲苯所致小鼠炎性耳肿实验和体外抑菌实验考察药物的抗炎效果和抑菌作用。结果与烫伤对照组相比,醇提蛋黄油能明显减少创面渗出、缩短结痂时间和愈合时间(P<0.01),作用效果与干馏蛋黄油相近,并优于湿润烧伤膏(P<0.05);与对照组相比,醇提蛋黄油具有显著抗炎作用(P<0.01),其作用效果类似干馏蛋黄油(P>0.05)并优于湿润烧伤膏(P<0.05);醇提蛋黄油的体外抑菌作用明显优于其他组,尤其对金黄色葡萄球菌抑制作用明显。结论醇提蛋黄油具有明显促进实验动物烫伤创面愈合的作用。

To study the effect of egg yolk oil by ethanol extract on scald wound and its anti-inflammation and its anti-bacteria effect in vitro. Methods Healthy Kunming mice of second degree scald were divided into the scalding control group, moisture burn ointment positive control group, dry distilled egg yolk oil treatment group and ethanol extracted egg yolk oil treatment group. The mice model with the grade II burn on the back were established, the healing time, the change of scald surface and the effects were observed. The experiment of inflammation caused by dimethyl-benzene on the ears of mice was carried out to observe the effect on anti-inflammation. The anti-bacteria effect of the sample was studied in vitro. Results Compared with that of negative control group, the egg yolk oil by ethanol extract could apparently decreased the wound extravasation and shorten healing time. It had the similar effect with that of dry distilled egg yolk oil, was better than that of moisture burn ointment. Compared with that of the control group, the egg yolk oil by ethanol extract had the significant anti-inflammatory effects（ P 〈0.01）. It had the similar effect with that of dry distilled egg yolk oil（ P〉0.05）, was better than that of moisture burn ointment （ P〈0. 05）. and inhibitory effect on bacteria was better than that of other groups, especially on Staphylococcus aureus. Conclusion Ethanol extract of egg yolk oil has obvious effect on promoting scald wound healing.