液相色谱法分析牛血清中IgG的方法研究

Study on the measure of analysis of IgG in bovine serum with HPLC

本文以牛布氏菌病血清为例,阐述了采用两种不同原理的高效液相色谱法测定血清中IgG含量的方法。通过实验,筛选出利用Protein G柱测定血清中IgG含量的方法比较理想,该方法精密度试验的相对标准偏差为0.2%,平均回收率为95.71%,回收率试验的相对标准偏差为0.9%。并利用该方法测定不同的牛布氏菌病阳性血清中IgG的含量,与其效价(补体结合试验测定)进行比较,可知在一定范围内牛布氏菌病阳性血清中的IgG含量与其效价存在一定的趋势性对应关系。

In this paper, the content of IgG in serum was evaluated by two HPLC methods, based on different principles. Comparing the methods, the better method was elected, RSD of degree of precision test is 0.2%, the average coefficient of recovery is 95.71%, RSD is 0.9%. The relationship of biological potency and content of IgG in serum was analyzed.