人脐带间充质干细胞治疗失代偿期肝硬化的疗效观察

Therapeutic effect of human umbilical cord mesenchymal stem cell transplantation on decompensated cirrhosis

目的观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUC-MSCs)治疗失代偿期肝硬化患者的疗效。方法 24例肝硬化失代偿期患者,经HUC-MSCs移植,于移植前、移植后1、2、4、8、12周检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBiL)、凝血酶原时间(PT)和白蛋白(ALB)水平。于移植前、移植后8、12周分别行CT检查,观察临床症状改善情况及不良反应。结果移植后2周各项肝功能指标无显著变化(P>0.05);移植后4周,ALT由(106±18)IU/L降至(82±12)IU/L,AST由(121±23)IU/L降至(87±14)IU/L,较移植前明显改善(P<0.05),其余指标无明显改善;移植后8周各项肝功能指标均有改善(P<0.05)。12周有显著改善(P<0.01)。临床症状随时间进展逐步改善且无不良反应。肝脏最大横截面积改善(P<0.05),但肝脏CT值无显著变化(P>0.05)。结论 HUC-MSCs移植可显著改善失代偿期肝硬化患者肝功能及临床症状,并能促进肝脏修复。

Objective To observe the therapeutic effect of human umbilical cord mesenchymal stem cell （HUC-MSC） transplantation on patients with decompensated cirrhosis. Methods ALT, AST, TBiL, PT, ALB levels were measured in 24 patients with decompensated cirrhosis before and 1, 2, 4, 8, 12 weeks after they underwent HUC-MSC transplantation. Their clinical symptoms and adverse reactions were observed by CT before and 8, 12 weeks after HUC-MSC transplantation. Results No significant improvement was found in liver function of the patients 2 weeks after HUC-MSC transplantation （P〉0.05）. The ALT and AST levels were significantly lower 4 weeks after HUC-MSC transplantation than before HUC-MSC transplantation （（82 ± 12）IU/L vs （106 ± 18）IU/L, （87 ± 14）IU/L vs （121 ± 23） IU/L, P〈0.05）. No significant improvement was observed in the other liver function indications of the patients. All the indications of liver function obviously improved 8 and 12 weeks after HUC-MSC transplantation （P〈0.05）. The clinical symptoms of the patients alleviated with the prolongation of time. No adverse reaction occurred. The liver maximum cross-sectional area was significantly larger after HUC- MSC transplantation （P〈0.05） with no significant change in CT value of liver （P〉0.05）. Conclusion HUC-MSC transplantation can significantly improve the fiver function and clinical symptoms of patients with decompensated cirrhosis and promote their liver repair.