瘦素受体基因Gln223Arg多态性与脑卒中相关性的研究

The study of Association between Leptin Receptor Gene Polymorphisms and Stroke

目的探讨瘦素受体(leptin receptor,LR)基因Gln223Arg位点多态性与脑卒中的相关性。方法采用聚合酶链反应-限制性片段长度多态性分析法检测50例脑卒中患者和50例正常对照者的LR基因Gln223Arg位点多态性,同时记录受试者年龄、性别,并测定血糖、胆固醇(CHO)、低密度脂蛋白(LDL)、收缩压和舒张压等临床指标进行对比分析。结果脑卒中组的瘦素受体基因G和A等位基因频率分别为74.0%和26.0%,正常对照组分别为84.0%和16.0%,两者比较差异有统计学意义(P<0.05)。与对照组基因型比较,基因型中含有等位基因A的各临床指标要高于对照组。结论 LR基因Gln223Arg位点的A等位基因会增加脑卒中的发病风险。

Objective To investigate the association of Gln223Arg polymorphism in leptin receptor gene with the risk of stroke.Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect the leptin receptor gene Gln223Arg polymorphism in 50 stroke patients and 50 normal controls.The age and sex of patients were recorded.Other clinical parameters,including glucose,cholesterol（CHO）,low density lipoprotein（LDL）,systolic pressure and diastolic pressure were determined and analyzed.Results The frequencies of G and A allele of leptin receptor gene in stroke group（75.0% and 25.0%） were significantly different from those in control group（84.0% and 16.0%,P〈0.05）.Compared with the genotype of the control group,the clinical parameters in genotypes containing allele A was higher than that in control group.Conclusions The allele frequencies of A in Gln223Arg gene correlated with the increased risk of stroke.