β_3肾上腺素能受体基因Trp64Arg多态性与蒙古族原发性高血压病相关性分析

Association of Beta 3 Adrenergic Receptor Gene Polymorphism and Essential Hypertension in Mongolian Population

目的检测蒙古族原发性高血压人群中β3肾上腺素能受体基因Trp64Arg多态性,探讨其与蒙古族人群原发性高血压病(EH)和其他心血管病危险因素的关系。方法应用PCR技术检测原发性高血压病患者102例,健康体检者93例。比较两组Trp64Arg突变基因型和临床特征。结果高血压病组与对照组β3-AR基因突变频率两者差异无统计学意义(P>0.05),基因Trp64Arg突变者的体质量指数显著高于正常基因型(P<0.05),突变者在三酰甘油、血糖、胰岛素、尿酸方面差异有统计学意义(P<0.05)。结论 Trp64Arg基因突变可能不是蒙古族原发性高血压病发生的决定因素,但该基因变异可能与肥胖、脂代谢、糖代谢等危险因素有关。

Objective To investigate the association of beta 3 adrenergic receptor（β3 AR）gene Trp64Arg polymorphism and essential hypertension （EH） in Mongolian population. Methods A total 207 Mongolian subjects including 102 patients with EH and 93 healthy controls were enrolled in the study. Polymerase chain reaction - restriction fragment length polymorphism （PCR - RFLP） and allele- specific （AS）-PCR assays were used to identify Trp64Arg genotypes. The genotypes and related clinical features were compared. Results The Mutation frequency of Trp64Arg in both groups were 33% and 33%,respectively（P〉0.05）. In the patients group,body mass index（BMI）in patients with gene Trp64Arg mutation were significantly higher than that in patients with normal genotype（P〈0.05）. Triglycerides, glucose, insulin, uric acid were significantly different between the two groups （P〈 0.05 ）. Conclusion Trp64Arg mutation may not be the determinants of the occurrence of hypertension. However,the gene mutation may be related to obesity,lipid metabolism,glucose metabolism and other risk factors.