两种肝再生模型肝切除术后肝再生指数和肝再生度的实验研究

Experimental research of liver regeneration and liver regeneration index after partial hepatectomy in two kinds of liver regeneration's models

目的探讨大鼠肝细胞增殖介导肝再生和卵圆细胞增殖介导肝再生两种不同大鼠肝再生模型肝切除术后肝再生指数和肝再生度变化的情况。方法 SD大鼠随机分为2组:肝细胞增殖介导肝再生模型组(PH),卵圆细胞增殖介导肝再生模型组(2AAF/PH)。计算两组模型肝切除术后4d、8d、12d、16d、20d残肝肝再生度及肝再生指数。结果两组肝再生度、肝再生指数均在第4天明显升高,12天左右达到高峰,超过再生总量的2/3以上,至20天基本达到原肝重。在4d、8d、12d天同一时间点内,PH组肝再生度比2AAF/PH组高(P<0.05),而到16d时,2AAF/PH组肝再生度则比PH组高(P<0.05)。在4d、8d天同一时间点内,PH组肝再生指数比2AAF/PH组高(P<0.05)。在12d、16d、20d同一时间点内,两组肝再生指数无显著性差异(P>0.05)。结论肝再生度与肝再生指数是评价肝再生质量变化较直观和准确的指标,肝再生度在反映肝再生规律方面要比肝再生指数更准确。

Objective To investigate the change of liver regeneration degree （LRD） and liver regeneration index （LRI） after partial hepatectomy in two different rats＇liver regeneration models. Methods SD rats were randomized into 2 groups： Liver regeneration models＇group mediated by hepatic cells proliferation（PH） and liver regeneration models＇group mediated by oval cells proliferation （2AAF/PH）. LRD and LRI were calculated in the time points of 4,8,12,16,20 days after liver resection. Results In both PH group and 2AAF/PH group, LRD and LRI of POD （post operation day） 4 were increased significantly, and reached the peak on POD12 and the weight of residual liver was more than two-thirds of the total amount of regeneration. On POD20, the proliferating residual liver almost reach the size of original liver. And LRD of PH group are higher than 2AAF/PH group on POD4,8,12 （P〈O.05）. But on POD16, LRD of 2AAF/PH group are higher than PH group （P〈 0.05）. On POD4,8, LRI of PH group are higher than 2AAF/PH group （P〈0.05） and no statistical difference on POD12,16,20 （P〉0.05）. Conclusions TO evaluate the change of liver regeneration quality, LRD and LRI are intuitive and precise. But LRD looks more accuracy than LRI in reflecting the liver regeneration.