摘要
目的研究同时阳性样本的乙型肝炎病毒(HBV)表面抗原(HBsAg)和乙型肝炎病毒表面抗体(抗-HBs)的体外中和反应特性。方法运用化学发光定量检测技术在HBsAg阳性样本中收集抗-HBs同时阳性的样本血清作为研究对象;选择单纯HBsAg阳性的样本和单纯抗-HBs阳性的样本分别与同时阳性的研究组样本中的抗-HBs和HBsAg进行体外血清学中和反应,测定中和物中的HBsAg和抗-HBs含量;测定HBsAg单阳性样本的基因型和HBsAg亚型,记录各测定结果。结果获取1例HBsAg(130.21ng/mL)和抗-HBs(50.6U/L)双阳性样本,HBV基因型为B型,HBsAg亚型为adw;20例HBsAg单阳性样本中有6例能中和研究组样本中的抗-HBs(中和物抗-HBs≤6.5 U/L);20例抗-HBs单阳性样本均能中和研究组样本中的HBsAg(中和物HBsAg≤0.06ng/mL)。结论同时阳性样本中的抗-HBs不是与其共存的HBsAg的特异性抗体。
Objective To study the neutralization reaction characteristics in vitro of HBsAg and anti-HBs co- exist positive samples. Methods Using chemiluminescent assay,the anti-HBs-positive serum samples were collected from serum HBsAg-positive samples as the research group. Simple HBsAg-positive samples and simple anti-HBs- positive samples were respectively selected and performed the serum neutralization reaction with anti-HBs and HB- sAg in HBsAg and anti-HBs coexist positive samples of the research group. The HBsAg and anti-HBs levels in the corrective were detected. The genotypes and HBsAg subtypes of simple HBsAg-positive samples were detected. The detection results were recorded. Results 1 case of coexist positive HBsAg（130.21 ng/mL） and anti-HBs（50.6 U/L） was detected. HBV genotype was B-type, HBsAg subtypes was adw. Among 20 simple HBsAg positive samples, 6 cases could completely neutralized anti-HBs existing in coexist positive samples, （corrective anti-HBs ≤6.5 U/L）. All 20 simple anti-HBs positive samples could completely neutralized HBsAg existing in coexist positive samples （HBsAg≤0.06 ng/mL）. Conclusion Anti-HBs in HBsAg and anti-HBs coexist positive samples is not the coexist HBsAg-specific antibodies.
出处
《检验医学与临床》
CAS
2012年第12期1409-1410,1412,共3页
Laboratory Medicine and Clinic
基金
湖南省第二人民医院重点专科基金资助项目(湖南省卫生厅2010年序号04)
