血型自动化检测在血站血型定型中的应用

Application of blood-group automation detecting in blood typing in blood stations

目的探讨分析全自动血型检测系统在血站血型检测中应用的可行性。方法使用Microlab Star8CH全自动加样系统加样、AK03A全自动数字血型分析仪判读,组成血型全自动化检测系统检测1 487份无偿献血者的ABO和Rh血型,从血型判读的符合率、混合血清样本倍比稀释后反定型检测灵敏度、溶血及脂血干扰试验4个方面进行分析。结果全自动血型检测系统血型判读符合率达99.7%;混合血清小于或等于1∶64的稀释度反定型检测准确度达100.0%,脂血和溶血标本对检测结果无影响。结论全自动血型检测技术具有较高的准确度,实现了血型检测标准化、自动化,适用于血站血型的批量检测。

Objective To discuss the application feasibility of automatic blood group detecting system in blood group check in the blood stations. Methods The Microlab Star 8CH full automation sampling system for adding sample and the fully automatic AK03A figure blood group analyser for interpretation were combined into the blood group full automation detecting system. This system was used to detect ABO and Rh blood group in 1 487 free blood donators. The analysis was performd in 4 aspects of the coincidence rate in blood group interpretation,the sensitivity of reverse typing detection after doubling dilution of mixed serum samples, hematolysis and lipidemia interference. Re- sults The coincidence rate interpreted by the blood group full automation detecting system reached 99.7%. The sen- sitivity of reverse typing detection of mixed serum dilution ≤ 1 ： 64 reached 100%. The lipidemia and hematolysis specimens had no influence to the test results. Conclusion The full automation blood group testing technique has higher accuracy and capacity, and realizes the standardization and automation of blood group test, which is suitable for the blood group batch detection in the blood stations.