哮喘大鼠TLR1、FasL和TRAF2表达及甲基强的松龙干预作用

Regulation with Methylprednisolone on the Expression of Toll like receptor 1, FasL, and Tumor Necrosis Factor Receptor Associated Factor 2 in Asthmatic Rats

目的:探讨TLR1、FasL和TRAF2在大鼠哮喘炎症机制中的作用,观察甲基强的松龙对其表达的影响。方法:27只SD大鼠,随机分成哮喘模型组、正常对照组和甲基强的松龙组,每组9只,采用流式细胞术检测血淋巴细胞TLR1和FasL表达,免疫组织化学法检测肺组织TRAF2表达。结果:哮喘组血淋巴细胞TLR1表达水平显著低于甲基强的松龙组(P<0.05),正常对照组血淋巴细胞TLR1表达水平分别与哮喘组及甲基强的松龙组比较,差异无统计学意义(P均>0.05)。哮喘组和甲基强的松龙组血淋巴细胞FasL表达水平均显著高于正常对照组(P均<0.05),而哮喘组血淋巴细胞FasL表达水平与甲基强的松龙组比较差异无统计学意义(P>0.05)。哮喘组TRAF2光密度值显著高于正常对照组和甲基强的松龙组(P均<0.01),甲基强的松龙组TRAF2光密度值与正常对照组比较,差异无统计学意义(P>0.05)。结论:哮喘模型大鼠FasL和TRAF2表达增加,而TLR1无变化;甲基强的松龙能下调TRAF2和提升TLR1水平,从而起到抗炎作用。

Objective：To investigate the potential effects of methylprednisolone on the expression of Toll like receptor 1（TLR1）, FasL, and tumor necrosis factor receptor associated factor 2（TRAF2） in asthmatic rats. Methods： Twenty-seven SD rats were randomly divided into 3 groups, including asthma group, control group, and methyl- prednisolone-treated group. The levels of TLR1 and FasL in blood lymphocyte were detected by flow cytometry. The expression of TRAF2 protein was detected by immunohistochemical methods. Results： The level of TLR1 in the asthma group（47.58±1.46 ） MFI was significantly lower than that in the methylprednisolone-treated group （52.89±3.92）MFI （P〈0.05）; The level of TLR1 in the control group did not differ to that in the asthma group or the methylprednisolone-treated group（all P〉0.05）. The level of FasL in the asthma group（15.52±0.53 MFI） and the methylprednisolone-treated group（14.46±2.19 MFI） were both significantly higher than that in the control group（11.73±1.39 MFI）（all P〈0.05）, but no significant difference was noted between the asthma group and the methylprednisolone-treated group（P〉0.05）. The expression of TRAF2 protein in the asthma group （0.317± 0.041 optical density） was dramatically higher than that in the control group（0.220±0.057 optical density）and the methylprednisolone treated group（0.235±0.028 optical density） （all P〈0.01）, but no significant difference was seen between the methylprednisolone-treated group and the control group （P〉0.05 ）. Conclusion： The levels of FasL and TRAF2 were elevated and TLR1 had no change in asthmatic rats. The anti-inflammation role of methylprednisolone may be partly through down-regulating TRAF2 and up-regulating TLR1, but had no effect on FasL.