小鼠ret中间片段真核表达载体的构建

Construction of ret Fragment's Eukaryotic Expression Vector

利用RT-PCR法从小鼠睾丸组织中获得ret的中间片段,首先将其连接到pMD18-T载体上,转化DH5α后通过阳性克隆子的鉴定以及序列测定,将序列正确的ret中间片段双酶切下来后连接到真核表达载体pcDNA3.1上。结果表明成功构建了ret中间片段的真核表达载体pcDNA3.1-ret。

The ret fragment was cloned by RT-PCR from mouse testis tissue,and linked to vector pMD18-T,which was used for transformation of DH5α afterward.The possitive clone was identified and sequenced.Expression vector pcDNA3.1-ret was then constructed with the double digested ret fragment.The result indicated that ret fragment’s eukaryotic expression vector pcDNA3.1-ret was constructed successfully.