双污泥反硝化聚磷系统污泥的培养与驯化

Culture and Enrichment of Denitrifying Phosphorus Removing Sludge and Nitrifying Activated Sludge

以污水处理厂氧化沟污泥为泥种,采用进水低碳高磷、两阶段的运行方式进行反硝化聚磷污泥的培养,约100d成功驯化培养出反硝化聚磷污泥。第1阶段以厌氧/好氧的运行方式驯化好氧聚磷污泥,运行约40d,最大释磷量、最大聚磷量和最大除磷量分别可达到77.2、89.4、25.0mg/L,表现出较强的聚磷能力;第2阶段采用厌氧/缺氧/好氧的运行方式驯化反硝化聚磷污泥,运行60d,缺氧聚磷量占总聚磷量的百分比呈上升趋势。硝化污泥经过100d的驯化可去除约50mg/L的氨氮,硝化率基本稳定在98.5%以上。硝化速率本符合零级动力学方程,比硝化速率常数为0.002 4h-1;好氧聚磷速率和缺氧聚磷速率基本符合一级动力学方程,速率常数分别是0.377、0.740g/(L.h-1)。利用驯化培养成功的反硝化聚磷污泥和硝化污泥进行了A2N-SBR试验,结果表明:在进水COD、氨氮和磷分别为188.0、54.8、7.25mg/L时,去除率分别为93.5%、76.7%和94.1%,驯化培养的双污泥具有良好的脱氮除磷效果。

In the experiment, the denitrifying phosphorus removing bacteria （DPB） was cultivated by high phosphorus and low carbon source influent. It was cultivated successfully by the 2-stage mode for about 100 d. The first stage was about 40 d, which was with the mode of anaerobic/aerobic to enrich the phosphorus accumulating organisms （PAOs）. The maximum amounts of the phosphorus release, phosphorus uptake and phosphorus removal were 77.2 mg/L, 89.4 mg/L and 25.0 mg/L respectively. The second stage was 60 d, which was with the mode of anaerobic/anoxic/aerobic. In this stage, the ratio of denitrifying phosphorus uptake amount to the total phosphorus uptake amount had an ascending tendency. The cultivation of nitrifying activated sludge was about 100 d, with the 50 mg/L ammonia removal amount and more than 98.5O/oo ammonia removal efficiency. Nitrification rate fitted zero order kinetic equation, and the specific nitrification rate constant was 0. 002 4 h-1 Phosphorus uptake rates fitted first order absorption kinetic equation. The uptake rate constants under aerobic and anoxic conditions were 0. 377 and 0. 740 L/（g · h 1）. The cultivated DPB sludge and nitrifying activated sludge were used in an anaerobic/ anoxic/nitrification-sequencing batch reactor（A2N- SBR） process to remove nitrogen and phosphorus. When the concentrations of influent COD, ammonia and phosphorus were 188.0 mg/L, 54. 8 mg/L and 7.25 mg/L, the removal efficiencies of COD, ammonia and phosphorus were 93.50/oo, 76.7% and 94.1% respectively.