以Fe(Ⅲ)为电子受体的聚磷菌筛选、鉴定及聚磷特性

Screening and Identification of Phosphate Accumulating Bacteria with Fe(Ⅲ) as Electronic Acceptor and its Effects on Phosphorus-Accumulating Characteristics

结合平板分离法、poly-P和PHB染色以及柠檬酸铁还原实验,从污水处理厂活性污泥中分离获得一株以Fe(Ⅲ)为电子受体厌氧聚磷的聚磷菌株AP3,其16SrDNA与Pseudomonas mosselii ATCCBAA-99的同源性为99%。菌株AP3于柠檬酸铁液体培养基和无铁液体培养基中厌氧避光、27℃静置培养,在含有Fe(Ⅲ)的培养基中比在无Fe(Ⅲ)的培养基中生长好;菌株AP3在无Fe(Ⅲ)的培养基中,表现出典型的厌氧释磷特征,释磷量为3.68mg.L-1;在含有Fe(Ⅲ)的培养基中,先厌氧释磷,释磷量为6.62mg.L-1,10h之后开始厌氧聚磷,聚磷量为5.89mg.L-1。菌株AP3能以Fe(Ⅲ)为电子受体,具有厌氧聚磷特征。

A phosphorus accumulation organism strain AP3 with Fe （ Ⅲ ） as electronic acceptor was screened from activated sludge through such processes as dilution-plate method, poly-P, PHB staining and ferric citrate reduction. Its 16S rDNA is identical to that of Pseudomonas mosseliisp ATCCBAA-99 at 99% level. By comparing the AP3 being cultured in ferric citrate fluid medium and the one in non-ferric fluid medium at 27 ℃, it was found that ferric citrate fluid medium was beneficial for the AP3. AP3 cultured in non-ferric fluid medium showed significant anaerobic and phosphorus releasing characteristic, the phosphorus releasing amount of which was 3.68 mg · L -1. AP3 cultured in ferric fluid medium was also characterized by anaerobic and phosphorus releasing property at the first stage, the phosphorus releasing amount of which was 6.62 mg · L -1 ,which was higher than the former; And at the second stage which started from the 10h, it was characterized by anaerobic and phosphorus accumulating property, the phosphorus accumulating amount of which was 5.89 mg·L-1. Fe （Ⅲ） is the electron acceptor of AP3 which shows typical anaerobic and phosphorus absorbing property.