大蒜素诱导骨肉瘤细胞凋亡的作用及其机制的实验研究

Effects and Mechanisms of Osteosarcoma Cell Apoptosis Induced by Allicin

目的探讨大蒜素诱导骨肉瘤细胞凋亡的作用及其机制。方法使用不同浓度大蒜素处理骨肿瘤细胞系后,采用流式细胞术检测细胞凋亡率,RT-PCR技术以及免疫印迹技术检测Bax的mRNA及蛋白水平的表达变化。结果大蒜素处理后骨肉瘤细胞凋亡率明显升高,并呈剂量依赖性;且细胞内的Bax的mRNA及蛋白表达均显著升高(P<0.05)。结论大蒜素在体外可诱导多种骨肉瘤细胞凋亡,可能与上调Bax的表达,促进细胞色素c的释放有关;大蒜素有望成为一种新的治疗骨肉瘤的化疗药物。

Objective To investigate the effects and mechanisms of osteosarcoma cell apoptosis induced by allicin. Methods Osteosarcoma cells were treated with different concentrations of allicin in vitro, the apoptotic rate was detected by flow cytometry, mRNA and protein expression of Bax were detected respectively by using RT-PCR and western blotting. Results The apoptotic rate of osteosarcoma cells was significantly increased in a dose-dependent manner after being treated with allicin. Both mRNA and protein expressions of Bax in osteosarcoma cells increased significantly （P 〈 0.05）. Conclusion Allicin was able to induce the apoptnsis of osteosarcoma cells through upregulating the expression of＇Bax in vitro. It may become a potential chemotherapeutic agent for the treatment of osteosarcoma.