槟榔壳多酚组分及抗氧化活性的测定(英文)

Determination of Polyphenols Ingredients Present in Areca Husk and Its Antioxidant Activity

建立测定槟榔壳中多种酚类物质的高效毛细管电泳方法,分析不同浓度和不同pH值硼酸缓冲液对10种标准品的分离效果,最后确定最佳缓冲液为0.1 mol/L,pH 9.0的硼酸缓冲液,紫外检测波长为280 nm,分离电压为20 kV。方法简便快速,能在20 min之内将10种酚类物质完全分离开,检测限为0.5～4.5 mg/L。此外,进一步测定了槟榔壳多酚的抗氧化活性,选用3个评价抗氧化能力的指标,即:对DPPH自由基的清除能力、对还原能力以及对ABTS自由基的清除能力。

This paper was developed for the simultaneous determination of betel nut shell in a variety of phenolic compounds by high performance capillary electrophoresis method, analyzed the separation effect of different concentrations and pH values of buffer on 10 analytes, finally determined the best electrolytic buffer was 0.1 mol/L, pH 9.0 borate buffer, UV detection wavelength 280 nm, separation voltage 20 kV. The method is simple and fast, within 20 min 10 kinds of phenolic materials can be completely separated, the detection limit is 0.5N4.5 mg/L. In addition, this article further determines of the betel nut shell polyphenol antioxidant activity, by using three evaluation of the antioxidant capacity ＇of index, namely： the DPPH free radical scavenging ability, reduction ability as well as ABTS free radical scavenging ability.