期刊文献+

槟榔壳多酚组分及抗氧化活性的测定(英文) 被引量:3

Determination of Polyphenols Ingredients Present in Areca Husk and Its Antioxidant Activity
下载PDF
导出
摘要 建立测定槟榔壳中多种酚类物质的高效毛细管电泳方法,分析不同浓度和不同pH值硼酸缓冲液对10种标准品的分离效果,最后确定最佳缓冲液为0.1 mol/L,pH 9.0的硼酸缓冲液,紫外检测波长为280 nm,分离电压为20 kV。方法简便快速,能在20 min之内将10种酚类物质完全分离开,检测限为0.5~4.5 mg/L。此外,进一步测定了槟榔壳多酚的抗氧化活性,选用3个评价抗氧化能力的指标,即:对DPPH自由基的清除能力、对还原能力以及对ABTS自由基的清除能力。 This paper was developed for the simultaneous determination of betel nut shell in a variety of phenolic compounds by high performance capillary electrophoresis method, analyzed the separation effect of different concentrations and pH values of buffer on 10 analytes, finally determined the best electrolytic buffer was 0.1 mol/L, pH 9.0 borate buffer, UV detection wavelength 280 nm, separation voltage 20 kV. The method is simple and fast, within 20 min 10 kinds of phenolic materials can be completely separated, the detection limit is 0.5N4.5 mg/L. In addition, this article further determines of the betel nut shell polyphenol antioxidant activity, by using three evaluation of the antioxidant capacity 'of index, namely: the DPPH free radical scavenging ability, reduction ability as well as ABTS free radical scavenging ability.
出处 《热带作物学报》 CSCD 北大核心 2012年第4期717-725,共9页 Chinese Journal of Tropical Crops
基金 中国热带农业科学院环境与植物保护研究所业务费:"红棕象甲寄主选择的生理生化机制研究(No.2011hzs1J013)" 海南省重点科技计划项目"高产槟榔新品种的选育及栽培关键技术研究(No.ZDXM20100024)"
关键词 槟榔壳 毛细管电泳 多酚 抗氧化活性 Areca husk Capillary electrophoresis Polyphenols Antioxidant activity
  • 相关文献

参考文献16

  • 1Wetwitayaklung P, Phaechamud T, Limmatvapirat C, et ol. The study of antioxidant capacity in various parts of A raca catechu L[J]. Naresuan University Journal. 2006, 14 ( 1 ): 1-14.
  • 2Lei D, Chan C P, Wang Y J, et ol. Antioxidative and antiplatelet effects of aqueous inflorescence Piper betel extract [J]. Agriculture and Food Chemistry Journal. 2003, 51 (7): 2 083-2 088.
  • 3Wang C K, Lee W H. Separation, characteristics and biological activities of phenolics in areca fruit[J]. Journal of Agriculture and Food Chemistry. 1996, 44(8): 2 014-2 019.
  • 4Lee K K, Cho J J, Choi J D. Anti-elastase and anti-hyaluronidase of phenolic substance from A reca catechu as a new anti-aging agent[J]. International Journal of Cosmetic Science, 2001,23 (6): 341-346.
  • 5Mujumder A M, Kapadi A.H, Pendse G S. Chemistry and pharmacology of betel nut Areca catecht Linn[J]. Jounal of Plant. Crops 1979(7): 69-92.
  • 6Mathew A G, Parpia H A B, Govindarajan V S. The nature of the complex proanthocyanidins[J]. Phytochemistry, 1969, 8 (8) : 1 543-1 547.
  • 7Nonaka G, Hsu F, Nishioka I. Structures of dimeric, trimeric and tetrameric procyanidins from Areca catechu L[J]. Journal of the Chemical Society, Chemical Communications, 1981(15): 781-783.
  • 8Wu Q L, Yang Y H, Simon J E. Qualitative and Quantitative HPLC/MS determination ofproanthocyanidins in Areca Nut (A reca catechu )[J]. Chemistry & Biodiversity, 2007, 4( 12): 2 817-2 826.
  • 9He H M, He J. Determining tannic acid content of areca flower by reversed-phase HPLC[J]. Chinese Journal of Modem Applied Phamr-- acy, 1996, 13(1): 19-21.
  • 10Alekseeva M A, Eller K I Arzamastsev A P. Determining polyphenolic components of common hop by reversed-phase HPLC[.. Pharmaceu- tical Chemistry Journal, 2004, 38 ( 12): 39-41.

同被引文献45

引证文献3

二级引证文献31

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部