中枢细胞外Ca^(2+)对应激性高血压降压作用的实验研究

Experimental Study on the Central Extra - Cellular Calcium Ions Induce Depressor Effect in Rats with Hypertension Stress

将28只大鼠分为对照组和电击组(生理盐水+氯化钙组;氯丙嗪+氯化钙组;阿霉素+氯化钙组)。用电击足底的方法使大鼠产生应激性高血压,侧脑室给药。结果:(1)侧脑室注射氯化钙可使应激性高血压大鼠明显降压、减慢心率(P<0.01);(2)钙调素拮抗剂—氯丙嗪,钙/钙调素依赖性蛋白激酶抑制刻—阿霉素,可明显减弱或阻断氯化钙的降压和减慢心率作用。结果提示:中枢细胞外ca^(2+)增加对应激性高血压大鼠的降压作用,可能与钙调素和钙/钙调素依赖性蛋白激酶有关。

Twenty eight rats were randomly divided into control group and foot -shock stress groups (the latter were subdivied into group of given physiological saline and calcium chloride; group of give chlorproma/ine (chlor). and calcium chloride;group of given adriamycin and calcium chloride). The blood pressure has been rising significantly by chronic foot - shock stress in rats. The drugs were injected into the lateral ventricular. Result-s:that isotonic solution of calcium chloride was microinjected into ventricular elicited a significant depressor effect and decline in heart rate in stress - induced hypertension rats( P <0. 01). These effects of calcium chloride was markly decreased or blocked after calmodulin antagonist chlor. or calcium ions/calmodulin - dependent protein ki nase(Ca2+ /CaM- PK) inhibitor adriamycin was injected into the ventricular. It is suggested that an increase in the central extra -cellular calcium ions induce depressor effect in rats with hypertension induced by stress,these effects may be related to the CaM and Ca2+/Cam- PK.