抗黄瓜灰霉病的枯草芽孢杆菌筛选及其抑菌活性的初步研究

Screening Bacillus subtilis against Botrytis cinerea on Cucumber and Preliminary Studies of its Inhibition Activities

结合涂布平板法和温室盆栽植株法,测定了41株枯草芽孢杆菌发酵液对灰霉病病原菌及黄瓜灰霉病的抑制效果,以此筛选出针对灰霉病的生防菌株.结果表明,抑菌效果最好的枯草芽孢杆菌为BS01和BS03.采用涂布平板法研究了菌株BS01和BS03抑菌活性,结果表明,抑菌效果均为50%时,BS03比BS01的菌体浓度低,得到抑菌效果最好的菌株BS03.采用温室盆栽植株法测试了菌株BS03的抑菌活性,结果表明,菌悬液比发酵滤液的抑菌效果好,并且在菌悬液处理黄瓜苗后的第3天抑菌活性最高.

By the combination of in-vitro plate assay and in-vivo leaf assay, the biocontrol activity of 41 Bacillus subtilis strains in-vitro over mycelial growth of B. cinerea, as well as their controlling efficacies over gray mold on cucumber leaves in pots were evaluated, so as to screen the strains which could inhibit the mycelial growth ofB. cinerea. The results indicated that B. subtilis BS01 and BS03 were identified to have the strongest ability to control the cucumber gray mold. While the cell concentration of both BS01 and BS03 had 50% of effect in plate assay, the cell concentration of BS03 was lower. But BS03 appeared to have better effect. The controling efficacy of BS03 in-vivo leaf assay revealed that the cell suspension displayed a higher control efficacy than supematant. The cell suspension was used on cucumber leaves. After two days, the pathogen was inoculated, and the inhibition activity was then the highest.