舒喘灵对环加氧酶PGI_2合成酶和TXA_2合成酶活性的影响

Effect of Salbutamol on Cyclooxygenase, PGI_2 and TXA_2 Synthetase Activities

取猪肺微粒体用体外方法观察了舒喘灵对花生四烯酸（ＡＡ）实验代谢中环加氧酶、前列腺素（ＰＧＩ_２）合成酶及血栓素 Ａ（ＴＸＡ_２）合成酶的作用。结果表明，当以 ＡＡ为底物反应时各组均无明显反应，而当以前列腺素内过氧化物（ＰＧＨ_２）为底物反应时，舒喘灵３ × １０^（－６）ｍｏｌ／Ｌ组６－酮基－ＰＧＦ_（１ａ）的生成量明显增加（Ｐ＜０．０５）。提示舒喘灵对ＡＡ代谢中环加氧酶和ＴＸＡ_２合成酶影响不大，而对ＰＧＩ_２合成酶的活性有促进作用，故使ＰＧＩ_２生成量增加，６－ｋｅｔｏ－ＰＧＦ_（１ａ）／ＴＸＡ_２比值增大，因此有利于纠正ＰＧＩ_２－ＴＸＡ_２的平衡失调。

The effects of Salbutamol on cyclooxygenase, prostaglandin (PG) I_2 synthetase and thromboxane (TX) A_2 synthetase during the metabolism of arachidoic acid (AA) were studied in vitro. The results showed that when AA was used as a substrate, the levels of 6-keto-PGF_(1a) and TXB_2 had no significant difference among the groups, but when PGH_2 was used as a substrate, the level of 6-keto-PGF_(1a) was increased significantly following the use of Salbutamol 3 X 10^(-6) mol/L. The results showed that Salbutamol could enhance the activity of PGI_2 synthetase, but had no significant influence on the activities of cyclooxygenase and TXA_2 synthetase. Therefore, the amount of PGI_2 was increased and the ratio of the amount of 6-keto-PGF_(1a) to TXB_2 was augmented by which the balance of PGI_2 and TXA_2 was corrected.