肝细胞胸腺内注入对大鼠异位小肠移植物的影响

Effects of the intrathymic injection of hepatic cells on the small intestinal allograft in rat

目的 研究大鼠胸腺内注入异体抗原对异体小肠移植物的影响。方法 在未用任何免疫抑制剂的情况下， 将ＤＡ 大鼠的肝细胞注入ＰＶＧ 大鼠的胸腺内，１ 周后行同种异体异位小肠移植。结果 ３ 周内胸腺中注入的同种异体肝细胞存活； 移植小肠遭强烈排斥， 受者死亡， 但胸腺内注入的同一供者的肝细胞仍存活。结论 胸腺内注入同种异体肝细胞不能诱导受者对同一供者小肠移植物的免疫耐受。

Objective To study effects of the intrathymic injection of allohepatic cells on intestinal allograft. Methods Adult male DA and PVG rats, 7 9 week old, were used as donors and recipients, respectively. The ectopic small bowel transplantations were performed one week after intrathymic injection of hepatic cells without use of any immunosuppressants. The surgical procedures were carried out in a such manner that portal vein was anastomosed with left renal vein by the cuff, aorta was anastomosed with superior mesenteric artery with its aortic patch. Results The donor liver cells could survive in the recipient thymus for 3 weeks. The small intestinal allograft showed severe rejection and donor died one week after intrathymic injection of hepatic cells, but the hepatic cells could survive within the thymus at this time. Conclusion The intrathymic injection of allohepatic cells could not induce immune tolerance of the recipient to the same donor small intestinal graft.