皮肤鳞状细胞癌组织中HPV_(16)E_6/E_7的检测

Detection of Human Papillomavirus Type 16E_6/E_7 in Cutaneous Squamous Cell Carcinoma.

目的 了解１６ 型人乳头瘤病毒Ｅ６／Ｅ７（ ＨＰＶ１６Ｅ６／ Ｅ７） 与皮肤鳞状细胞癌的关系。方法 原位杂交及免疫组织化学染色（ＳＡＢＣ） 。结果 ６ 例患者的癌细胞中检测到ＨＰＶ１６ Ｅ６／Ｅ７ ，阳性信号主要出现在癌细胞的胞浆中。结论ＨＰＶ１６ Ｅ６／ Ｅ７ 可能在皮肤鳞癌的发病中具有重要作用。

Objective To investigate the relation of human papillomavirus type 16 E 6/E 7 (HPV 16 E 6/E 7) and cutaneous squamous cell carcinoma.Methods With immunohistochemistry (Strepavidin Biotin Complex) and in situ hybridization techniques.Results HPV 16 E 6/E 7 was detected in 6 of 8 cases.the positive signal was observed mainly in cytoplasm of cancer cells.Conclusion HPV 16 E 6/E 7 may play an important role in the carcinogenesis of cutaneous squamous cell carcinoma.