基因重组人碱性成纤维细胞生长因子的遗传毒性研究

Studied on Genotoxicity of Recommbinat Human Basic Fibroblast Growth Factor

用Ames试验、CHL细胞染色体畸变试验、小鼠骨髓PCE微核试验和小鼠致畸试验对基因重组人碱性成纤维细胞生长因子 (rh bFGF)进行研究。结果显示 ,rh bFGF(0 1～500μg/皿 )对TA97、TA98、TA100和TA102菌株在±S9mix条件下无致突变作用。各剂量组的微核细胞率与溶剂组比较 ,P>0 05。4个剂量组CHL细胞染色体畸变率均<5 %。在妊娠母鼠6～15dP0给药 ,各剂量组活胎率90 2 %～95 9 % ,与溶剂组95 %比较 ,P>0 05。对胎鼠外观、骨骼和内脏无致畸作用。但0 03264mg/kgb w吸收胎与溶剂组比较,P<0 01,显示一定的胚胎毒性。

The genotoxicity of recombinant human basic fibroblast growth factor(rh bFGF)was studied by Ames test,Chromosome aberration assay of mammalian cell(CHL)in vitro,mouse marrow micronucleus assay and teratogenesis assay.The results showed that rh-bFGF at the dose level of(0 1～500μg/plate)did not induce positive mutations in TA97,TA98,TA100,TA102 with or without S9mix of Ames test.It was control within the range(<5%)of chromosome aberration rates from rh bFGF with 4 doses groups with or without S9min.The frequency of mouse micronuleus rate had no increase.It was divided into each groups of rh bFGF received(p o)dosages the 6th to 15 day of gestation mouse respectively in the teratology test.The frequency of live fetuses of each dose was between 90 2～95 9%,there were no significant difference as compared with 95% of solution control group.It did not cause deformity of the fetus appearances,bone and internal organs.But there were significant differences from solution control in rf bFGF group of absorfoetus at 0 03264mg/kg(P<0 01).The results showed certainly dembyotoxicity.