鶲科五种鸟线粒体DNA序列变化与亲缘关系的研究

Study of the Relationship Among Five Species of Muscicapidae by mtDNA Sequence

首次对科 (Muscicapidae)画眉亚科三种鸟———画眉 (Garrulaxcanorus)、红嘴相思鸟 (Leiothrixlutea)、棕头雅雀(Paradoxorniswebbiana) ;鸫亚科的乌鸫 (Turdinaemerula)线粒体DNA的12SrRNA基因片段的DNA序列进行了测定 ,并与北美画眉亚科弯嘴鹛属 (Pomatostomus)的灰冠弯嘴鹛 (P.temporalis)同源序列进行分析比较并构建分子进化树。实验结果与传统形态学论述存在一定的差异,与同工酶研究结果相同 :画眉与红嘴相思鸟的亲缘关系最近 ,棕头鸦雀较乌鸫与画眉亚科的关系更近 ,为将其置于画眉亚科提供了分子证据。

The classfication on Muscicapinae disturbed us for a long time.The important thing is to find an appropriate character to resolve it.It is the first time that we testified the mitochondrial 12S rRNA gene fragment sequence of four birds (G.canorus,L.lutea,P.webbianus and T.merula owing to Muscicapinae) and aligned with the same sequence of P.temporalis.Using the mitochondrial 12S rRNA sequences date,we built phylogenetic trees.The results indicated that that the relationship between Garrulax canorus and Leiothrix lutea in the four birds was closer which is congruent with morphology.According to the results of isoenzyme and DNA sequencing data,we can draw a conclusion that Paradoxornis webbianus has closer relationship to Timaliinae than that of Turdus merula,and that putting Paradoxonithinae to Timaliinae was reasonable.