依托咪酯与牛血清白蛋白共振散射光谱的研究及其应用

Study and application of resonance light scattering in etomidate and bovine serum albumin system

目的:基于依托咪酯对牛血清白蛋白(BSA)的共振光散射增强作用,建立了测定依托咪酯的共振光散射法。方法:在pH=3.8 HAc-NaAc缓冲溶液中,依托咪酯使体系的共振散射光急剧增强,在468 nm处体系△IRLS值与依托咪酯的浓度呈线性关系,据此建立测定依托咪酯的新方法。结果:体系△IRLS值与依托咪酯的浓度在1.2μmol/L～35.0μmol/L范围内线性关系良好,线性回归方程为△IRLS=31+81.94c(μmol/L),r=0.997,检出限为0.37μmol/L,样品加标回收率为98.0～102.4%。结论:该方法简便,灵敏度高。用于样品中依托咪酯的测定,结果满意。

Objective：A new method was established for the determination of etomidate（ETM） with resonance light scattering based on ETM-BSA system.Methods：The ETM was used to enhance the intensity of resonance light scattering system in HAc-NaAc buffer solution（pH3.8）.Due to intensity of RLS was linear with the concentration of ETM at 468 nm,the method for determination of ETM was established.Results：The intensity of RLS had good linearity with the concentration of etomidate in the range of 1.2 μmol/L～35.0 μmol/L.The linear equation was △IRLS=31＋81.94c（μmol/L） with a correlation coefficient of 0.997.The detection limit was 0.37 μmol/L.The recoveries were between 98.0%～102.4%.Conclusion：The method is simple and sensitive.It has been applied to the determination of etomidate in samples with satisfactory results.