反相高效液相色谱法测定佛手散醇提物中川芎嗪、阿魏酸的方法学建立

Methodology for determination of tetramethylpyrazine and ferulic acid in ethanol extract of fo-shou powder by RP-HPLC

目的:建立佛手散乙醇提取物中的川芎嗪、阿魏酸方法学,为该方有效部位的药效学研究提供依据。方法:采用高效液相色谱法测定,川芎嗪及阿魏酸色谱条件分别为:甲醇-水-冰醋酸(35∶65∶0.5,v∶v∶v),柱温:25℃,检测波长:290 nm;甲醇-水-冰醋酸(27∶73∶0.5,v∶v∶v),柱温:30℃,检测波长:320 nm;计算两者方法回收率,考察精密度并进行重现性、稳定性实验。结果:川芎嗪在2μg/ml～64μg/ml线性范围内呈良好线性关系(r=0.9996),RSD为3.75%;阿魏酸在0.08μg/ml～2.56μg/ml线性范围内,线性范围内呈良好线性关系(r=0.9997),RSD为4.57%。结论:RP-HPLC对佛手散醇提物中川芎嗪、阿魏酸的测定专一性强,灵敏度高,样品较稳定,精密度和回收率好,操作简便易行,方法可靠。

Objective：To establish methodology for determination of the TMP,ferulic acid in ethanol extract of fo-shou powder and provide the basis for its pharmacodynamics research.Methods：TMP chromatographic conditions：methanol-water-acetic acid（35：65：0.5,v：v：v）,column temperature：25℃,detection wavelength：290 nm;Ferulic acid：methanol-water-acetic acid（27：73：0.5,v：v：v）,column temperature：30℃;detection：320 nm;Both recovery rates and precision were calculated,and the repeatability and stability were studied byRP high-performance liquid chromatography.Results：TMP in the linear range of 2 μg/ml～64 μg/ml showed good linear relationship（r = 0.9996）,RSD 3.75%;while ferulic acid in the linear range of 0.08 μg/ml ～2.56 μg/ml showed good linearity（r = 0.9997）,RSD was 4.57%.Conclusion：RP-HPLC had strong specificity,high sensitivity,stability,good recovery and precision,easy operation and reliability in determination of tetram-ethylpyrazine and ferulic acid in ethanol extract of fo-shou powder.