TRUST和TP-EIA法联合检测在梅毒血清学诊断的临床价值

Clinical value of combined detection of TRUST and TP-EIA in syphilis serological diagnosis

目的:检测梅毒的血清学方法很多,通过比较找出简单、快速适合临床需要的最佳的梅毒血清学实验。方法:对9865例病人血清标本分别用TRUST法和TP-EIA法进行梅毒抗体的检测,阳性者再用TPPA进行确证。结果:9865例血清标本TRUST法的阳性率为2.33%,TP-EIA法阳性率为2.92%,阳性者均经TPPA确证;进一步分析表明:TRUST阳性标本的TP-EIA的光密度值90%以上>1.0;而TRUST阴性标本的TP-EIA的光密度值90%以上<1.0;而且TRUST的滴度随病程变化而变化,而TP-EIA则不随病程变化而改变。结论:同时采用TRUST和TP-EIA进行血清学联合检测,阳性者再经TPPA确认,这样可互补两实验不足,提高阳性符合率,更好辅助临床诊断及疗效观察。

Objective：To find a optimal method for rapid syphilis serological diagnosis.Methods：Serum samples from 9865 patients were collected for syphilis antibody detection by TRUST and TP-EIA,then the positive cases were confirmed by TPPA.Results：The positive rate of 9865 serum samples was 2.33% by TRUST,2.92% by TP-EIA.The positive samples were all further confirmed by TPPA,which showed that optical density of TP-EIAin TRUST-positive samples was greater than 1.0 with the proportion of 90%,while that in TRUST-negative samples was lower than 1.0 with the same proportion.Titer of TRUST changed with the development of disease,while that of TP-EIA did not.Conclusion：Combined detection of TRUST and TP-EIA,and TPPA further confirmationcan improve the positive coincidence rate of samples,providing more help for clinical diagnosis and curative effect observation.