凝集素微量离心柱法检测甲胎蛋白异质体在肝癌诊断中的价值

Value of alpha fetoprotein isoforms in diagnosis of hepatocellular carcinoma with ACSC

目的:探讨凝集素微量离心柱法(lens culinaris agglutinin(LCA)-coupled spin column,ACSC)法检测甲胎蛋白异质体(alpha-fetoprotein isoforms,AFP-L3)对甲胎蛋白(alpha-fetoprotein,AFP)阳性的良恶性肝病鉴别诊断价值。方法:采用凝集素微量离心柱法及电化学发光法检测92例原发性肝癌(hepatocellu-lar carcinoma,HCC),45例慢性肝炎及肝硬化患者血清中AFP及AFP-L3浓度,以AFP-L3%≥10%为阳性判断标准,分析AFP-L3%与良恶性肝病的关系。结果:ACSC法检测AFP-L3%对92例原发性肝癌(HCC)的灵敏度是85.86%,对45例慢肝、肝硬化的特异性为95.56%,诊断准确度为89.05%,在受试者工作特征曲线(receiver operatin characteristic curve,ROC)曲线下的面积为0.9226。结论:ACSC法检测AFP-L3指标在PHC鉴别诊断中具有临床价值。

Objective：To investigate the diagnostic value of alpha-fetoprotein isoforms with lens culinaris agglutinin（LCA）-coupled spin column（ACSC） in differential diagnosis between benign liver disease and the liver malignancy.Methods：ACSC and electrochemiluminescence were used to detect the level of AFP and AFP-L3 in serum of 92 cases with hepatocellular carcinoma and 45 cases with chronic hepatitis and cirrhosis.The association of AFP-L3 level with the benign liver disease and the liver malignancy was analyzed according to the criterion on that AFP-L3≥10% was regarded as positive.Results：The sensitivity of AFP-L3 detected with ACSC in 92 HCCs was 85.86% and the specificity in 45 cases with chronic hepatitis and cirrhosis was 95.56%.the diagnostic accuracy was 89.05%.The area under the receiver operatin characteristic（ROC） curve was 0.9226.Conclusion：AFP-L3 detection with ACSC had clinical value in the diagnosis of primary hepatocellular carcinoma.