半夏泻心汤不同配伍情况下黄芩甙煎出量的RP-HPLC测定

Determination of Baicalin in Banxiaxiexintang Decoction Different Combinations of the by PR-HPLC

目的 :测定半夏泻心汤不同配伍情况下各组合中黄芩甙的煎出量。方法 :用YWGC18柱 ,甲醇 水 磷酸( 4 7∶5 3∶0 .0 5 )为流动相 ,紫外检测波长 2 74nm。结果 :复方按药物性味分组 ,各组间不同组合对黄芩甙煎出量有不同影响。结论 :该方法是以中医理论为指导 ,对复杂复方进行研究的有益尝试。

Objective:To the amount of baicalin in Banxiaxiexintang Decocted of differernt combinations were determined by PR HPLC.Method:An YWG C 18 column was used with a mobile phase of MeOH H 2O H 3PO 4(47∶53∶0.05) and an UV detector at the wavelength of 274 nm.Result and Conclusion:The amount of baicalin decreases when Radix Scutellariae is decocted with Rhizoma Coptidis, Rhizoma Pinelliae and Rhizoma Zingiberis. [